TY  - JOUR
AU  - Moter, Alina
AU  - Scharf, Sonja
AU  - Schäfer, Hendrik
AU  - Bexte, Tobias
AU  - Wendel, Philipp
AU  - Donnadieu, Emmanuel
AU  - Hansmann, Martin-Leo
AU  - Hartmann, Sylvia
AU  - Ullrich, Evelyn
TI  - Migration Dynamics of Human NK Cell Preparations in Microchannels and Their Invasion Into Patient-Derived Tissue.
JO  - Journal of cellular and molecular medicine
VL  - 29
IS  - 7
SN  - 1582-1838
CY  - Hoboken, NJ
PB  - Wiley-Blackwell
M1  - DKFZ-2025-00677
SP  - e70481
PY  - 2025
AB  - Natural killer (NK) cells are characterised by their ability to attack cancer cells without prior antigen stimulation. Additionally, clinical trials revealed great potential of NK cells expressing chimeric antigen receptors (CARs). Successful anti-tumour efficacy remains limited by migration and infiltration to the tumour site by NK cell preparations, which is linked to the scarcity in the knowledge of migration dynamics and invasion potential. Here, we applied a recently reported innovative microfluidic microchannel technology to gain insight into the intrinsic motility of NK cells. We assessed the baseline activated and proliferating NK cells in direct comparison with T cells and investigated their motility patterns in the presence of tumour cells. Additionally, we performed high-resolution 4D confocal imaging in patient-derived hyperplastic lymphatic tissues to assess their invasive capacity. Our data revealed that the invasion potential of NK cells was greater than that of T cells, despite their similar velocities. The flexibility of the NK cell nucleus may have contributed to the higher invasion potential. The motility of CD19-CAR-NK cell preparations was similar to that of non-transduced NK cells in hyperplastic lymphoid tissue, with improved targeted migration in tumour tissue, suggesting the suitability of genetically engineered NK cells for difficult-to-reach tumour tissues.
KW  - Humans
KW  - Killer Cells, Natural: immunology
KW  - Cell Movement
KW  - T-Lymphocytes: immunology
KW  - Receptors, Chimeric Antigen: metabolism
KW  - Receptors, Chimeric Antigen: immunology
KW  - Receptors, Chimeric Antigen: genetics
KW  - Lymphoid Tissue: immunology
KW  - Cell Proliferation
KW  - Lymphocyte Activation: immunology
KW  - Neoplasms: immunology
KW  - Neoplasms: pathology
KW  - Neoplasms: therapy
KW  - CAR‐NK (Other)
KW  - DLBCL tissue (Other)
KW  - NK cells (Other)
KW  - T cells (Other)
KW  - hyperplastic lymphoid tissue (Other)
KW  - microchannel (Other)
KW  - motility (Other)
KW  - Receptors, Chimeric Antigen (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40159644
DO  - DOI:10.1111/jcmm.70481
UR  - https://inrepo02.dkfz.de/record/300200
ER  -