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@ARTICLE{Koll:300253,
      author       = {K. K. Koll and D. A. Klevansky and D. Kasakovski$^*$ and N.
                      Ahmadzadeh and P. A. Will and U. Kneser and C. Hirche},
      title        = {{A}nalysis of the (lymph-)angiogenic potential of
                      regenerative scaffolds and the impact of adipose-derived
                      mesenchymal stem cells.},
      journal      = {Plastic and reconstructive surgery},
      volume       = {nn},
      issn         = {0032-1052},
      address      = {Philadelphia, Pa.},
      publisher    = {Lippincott Williams $\&$ Wilkins},
      reportid     = {DKFZ-2025-00714},
      pages        = {nn},
      year         = {2025},
      note         = {ISSN 1529-4242 / Division of Vascular Oncology and
                      Metastasis / DKFZ-ZMBH Alliance},
      abstract     = {Regenerative matrices are essential in the therapy of
                      complex wounds, where lymphangiogenesis and angiogenesis
                      play crucial roles. This work investigates the lymph- and
                      angiogenic potential of regenerative scaffolds and the
                      influence of adipose-derived mesenchymal stem cells (ADSC)
                      in a rodent wound model.In a wound model, we administered
                      different Acellular Dermal Matrices (ADMs) to two wounds on
                      each rat-enriching one with ADSCs and leaving the other
                      without. ADSCs were isolated from rodent inguinal fat
                      tissue, characterized using FACS, and their viability
                      confirmed in vitro on different matrices. Tissue samples
                      were collected on days 7, 14, 21, and 28 postoperatively.
                      Six different ADMs, including two synthetic and four
                      biological variants, were compared using histological,
                      immunofluorescence staining, and molecular biological
                      analyses.ADM integration varied, with Collagen-Elastin-ADM
                      showing the highest integration $(95\%)$ and
                      Polyurethane-ADM the lowest $(23\%)$ after 7 days. Synthetic
                      ADMs, particularly Polyurethan-ADM, exhibited the highest
                      mRNA expression of angiogenesis and lymphangiogenesis
                      markers at 7 and 28 days. Protein analysis showed synthetic
                      ADMs had the highest CD31 levels from 14 to 28 days, while
                      Collagen-ADM had the highest LYVE1 and PROX1 levels at 21
                      and 28 days. Immunofluorescence revealed Polylactide-ADM
                      consistently had the highest CD31 and LYVE1 signals at
                      various times, with Polyurethan-ADM and Collagen-ADM peaking
                      at 28 days. The application of ADSCs did not enhance
                      lymphangiogenesis or angiogenesis.ADM material properties
                      impact lymphangiogenesis and angiogenesis, guiding
                      therapeutic selection and tissue regeneration. This study
                      highlights Collagen-, Polylactide-, and Polyurethane-ADMs as
                      the most effective in promoting these processes.},
      keywords     = {Acellular dermal matrices (Other) / adipose-derived
                      mesenchymal stem cells (ADSC) (Other) / angiogenesis (Other)
                      / lymphangiogenesis (Other) / scaffolds (Other)},
      cin          = {A190},
      ddc          = {610},
      cid          = {I:(DE-He78)A190-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40178869},
      doi          = {10.1097/PRS.0000000000012131},
      url          = {https://inrepo02.dkfz.de/record/300253},
}