TY  - JOUR
AU  - Bertacchi, Michele
AU  - Theiß, Susanne
AU  - Ahmed, Ayat
AU  - Eibl, Michael
AU  - Loubat, Agnès
AU  - Maharaux, Gwendoline
AU  - Phromkrasae, Wanchana
AU  - Chakrabandhu, Krittalak
AU  - Camgöz, Aylin
AU  - Antonaci, Marco
AU  - Schaaf, Christian Patrick
AU  - Studer, Michèle
AU  - Laugsch, Magdalena
TI  - Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo.
JO  - Communications biology
VL  - 8
IS  - 1
SN  - 2399-3642
CY  - London
PB  - Springer Nature
M1  - DKFZ-2025-00752
SP  - 594
PY  - 2025
AB  - As a transcription factor, NR2F1 regulates spatiotemporal gene expression in the nucleus particularly during development. Aberrant NR2F1 causes the rare neurodevelopmental disorder Bosch-Boonstra-Schaaf Optic Atrophy Syndrome. In addition, altered NR2F1 expression is frequently observed in various cancers and is considered a prognostic marker or potential therapeutic target. NR2F1 has been found in both the nucleus and nucleoli, suggesting a non-canonical and direct role in the latter compartment. Hence, we studied this phenomenon employing various in vitro and in vivo models using different antibody-dependent approaches. Examination of seven commonly used anti-NR2F1 antibodies in different human cancer and stem cells as well as in wild type and null mice revealed that NR2F1 nucleolar localization is artificial and has no functional role. Our subsequent comparative analysis demonstrated which anti-NR2F1 antibody best fits which approach. The data allow for correct data interpretation and underline the need to optimize any antibody-mediated technique.
KW  - Humans
KW  - Animals
KW  - Cell Nucleolus: metabolism
KW  - Mice
KW  - COUP Transcription Factor I: metabolism
KW  - COUP Transcription Factor I: genetics
KW  - COUP Transcription Factor I: immunology
KW  - Mice, Knockout
KW  - Antibodies
KW  - Cell Nucleus: metabolism
KW  - Cell Line, Tumor
KW  - COUP Transcription Factor I (NLM Chemicals)
KW  - NR2F1 protein, human (NLM Chemicals)
KW  - Antibodies (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40204944
DO  - DOI:10.1038/s42003-025-07985-1
UR  - https://inrepo02.dkfz.de/record/300302
ER  -