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@ARTICLE{Bertacchi:300302,
      author       = {M. Bertacchi and S. Theiß and A. Ahmed and M. Eibl and A.
                      Loubat and G. Maharaux and W. Phromkrasae and K.
                      Chakrabandhu and A. Camgöz$^*$ and M. Antonaci and C. P.
                      Schaaf and M. Studer and M. Laugsch},
      title        = {{U}nravelling the conundrum of nucleolar {NR}2{F}1
                      localization using antibody-based approaches in vitro and in
                      vivo.},
      journal      = {Communications biology},
      volume       = {8},
      number       = {1},
      issn         = {2399-3642},
      address      = {London},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2025-00752},
      pages        = {594},
      year         = {2025},
      abstract     = {As a transcription factor, NR2F1 regulates spatiotemporal
                      gene expression in the nucleus particularly during
                      development. Aberrant NR2F1 causes the rare
                      neurodevelopmental disorder Bosch-Boonstra-Schaaf Optic
                      Atrophy Syndrome. In addition, altered NR2F1 expression is
                      frequently observed in various cancers and is considered a
                      prognostic marker or potential therapeutic target. NR2F1 has
                      been found in both the nucleus and nucleoli, suggesting a
                      non-canonical and direct role in the latter compartment.
                      Hence, we studied this phenomenon employing various in vitro
                      and in vivo models using different antibody-dependent
                      approaches. Examination of seven commonly used anti-NR2F1
                      antibodies in different human cancer and stem cells as well
                      as in wild type and null mice revealed that NR2F1 nucleolar
                      localization is artificial and has no functional role. Our
                      subsequent comparative analysis demonstrated which
                      anti-NR2F1 antibody best fits which approach. The data allow
                      for correct data interpretation and underline the need to
                      optimize any antibody-mediated technique.},
      keywords     = {Humans / Animals / Cell Nucleolus: metabolism / Mice / COUP
                      Transcription Factor I: metabolism / COUP Transcription
                      Factor I: genetics / COUP Transcription Factor I: immunology
                      / Mice, Knockout / Antibodies / Cell Nucleus: metabolism /
                      Cell Line, Tumor / COUP Transcription Factor I (NLM
                      Chemicals) / NR2F1 protein, human (NLM Chemicals) /
                      Antibodies (NLM Chemicals)},
      cin          = {B062 / HD01},
      ddc          = {570},
      cid          = {I:(DE-He78)B062-20160331 / I:(DE-He78)HD01-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40204944},
      doi          = {10.1038/s42003-025-07985-1},
      url          = {https://inrepo02.dkfz.de/record/300302},
}