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@ARTICLE{Petersen:300328,
author = {S. Petersen and L. Nagel and P. Groß$^*$ and H. de
Maissin$^*$ and R. Willing$^*$ and L. Heß$^*$ and J.
Mitschke and N. Klemm and J. Treiber and C. A. Müller and
S. Knecht and I. Schwartz and M. Weigt and M. Bock$^*$ and
D. von Elverfeldt$^*$ and M. Zaitsev$^*$ and E. Y. Chekmenev
and J.-B. Hövener and A. Martins$^*$ and F. Schilling$^*$
and T. Reinheckel$^*$ and A. Schmidt$^*$},
title = {{I}n vivo molecular imaging of breast cancer metabolic
heterogeneity using [1-13{C}]pyruvate-d3 hyperpolarized by
reversible exchange with parahydrogen.},
journal = {Theranostics},
volume = {15},
number = {9},
issn = {1838-7640},
address = {Wyoming, NSW},
publisher = {Ivyspring},
reportid = {DKFZ-2025-00774},
pages = {3714 - 3723},
year = {2025},
abstract = {Metabolic MRI using hyperpolarized (HP) [1-13C]pyruvate is
promising for diagnostic medicine, allowing the study of
cancer metabolism and early detection of therapy response.
However, a possible widespread and routine use requires
high-throughput and user-friendly technologies to produce
the hyperpolarized media. Methods: Recently, we introduced a
fast (6 min) and cost-effective method using Spin-Lock
Induced Crossing and Signal Amplification By Reversible
Exchange (SLIC-SABRE) at µT fields and a rapid purification
to produce biocompatible HP solutions of aqueous pyruvate.
In this study, we used SLIC-SABRE to conduct in vivo tumor
metabolic imaging in a transgenic breast cancer mouse model
(MMTV-PyMT). Results: In agreement with previous HP MRI
cancer studies, an elevated lactate metabolism was found in
tumors compared to healthy breast tissue, heart, and
vasculature, as well as distinct metabolic profiles within
different tumor compartments. These findings suggest a
potential link between lactate-to-pyruvate ratios and the
varying levels of tumor cell proliferation and apoptosis
observed by histological analyses. Conclusion: Our results
underscore the potential of SABRE to enhance the
accessibility and throughput of HP MRI, thereby advancing
cancer research and diagnostic oncology.},
keywords = {MMTV-PyMT (Other) / SABRE (Other) / hyperpolarization
(Other) / metabolic imaging (Other) / parahydrogen (Other)},
cin = {FR01 / TU01 / MU01},
ddc = {610},
cid = {I:(DE-He78)FR01-20160331 / I:(DE-He78)TU01-20160331 /
I:(DE-He78)MU01-20160331},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40213665},
pmc = {pmc:PMC11980672},
doi = {10.7150/thno.103272},
url = {https://inrepo02.dkfz.de/record/300328},
}