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@ARTICLE{Kumar:300356,
      author       = {R. Kumar and F. J. Hartmann$^*$ and P. Favaro and D. Ho and
                      T. Bruce and M. Goldston and A. Spence and E. F. McCaffrey
                      and S. C. Bendall and M. Angelo},
      title        = {{N}ew {A}tomic {M}ass {T}ags for {E}nhanced {M}ultiplexing
                      {C}apability of {M}ultiplexed {I}on {B}eam {I}maging
                      {T}ime-of-{F}light ({MIBI}-{TOF}) {A}nalysis.},
      journal      = {Analytical chemistry},
      volume       = {97},
      number       = {15},
      issn         = {0003-2700},
      address      = {Columbus, Ohio},
      publisher    = {American Chemical Society},
      reportid     = {DKFZ-2025-00790},
      pages        = {8220-8230},
      year         = {2025},
      note         = {2025 Apr 22;97(15):8220-8230},
      abstract     = {Antibodies conjugated to metal chelating polymers are
                      routinely used in high-dimensional multiplexed single cell
                      mass spectrometric imaging techniques, such as
                      immunohistochemistry-based multiplexed ion beam imaging by
                      time-of-flight (MIBI-TOF) mass spectrometry, imaging mass
                      cytometry (IMC), and flow cytometry-based CyTOF. However,
                      successful multiplexed capability of these techniques is
                      heavily dependent on the stability of the metal-chelates
                      used. Chelate stability is governed by the ionic radius of
                      the metal used, which in some cases can fall below or exceed
                      the optimal range for commercially available DTPA-based
                      polymers. In this study, we have developed and optimized
                      macrocyclic chelators for metals with relatively small
                      (i.e., Ga) or large (i.e., Tl) atomic radii. In agreement
                      with previously published studies, we observed NOTA to be a
                      suitable chelator for Ga, whereas DOTA was found to be an
                      ideal chelator for Tl and larger lanthanides, such as La,
                      Ce, and Pr. DOTA and DTPA chelator dendrimers were
                      synthesized and conjugated to primary antibodies that were
                      subsequently used for tissue staining. Antibodies conjugated
                      with the DOTA-dendrimer were more stable and exhibited more
                      specific staining than those modified with the corresponding
                      DTPA-dendrimer. With these new chelates, we incorporated
                      seven new reporter channels into a highly multiplexed
                      MIBI-TOF imaging study containing 44 protein epitope markers
                      on various tissues. To the best of our knowledge, this is
                      the largest multiplexed panel used to date for MIBI-TOF
                      applications.},
      cin          = {D260},
      ddc          = {540},
      cid          = {I:(DE-He78)D260-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40223204},
      doi          = {10.1021/acs.analchem.4c04300},
      url          = {https://inrepo02.dkfz.de/record/300356},
}