TY  - JOUR
AU  - Daugelaite, Klaudija
AU  - Lacour, Perrine
AU  - Winkler, Ivana
AU  - Koch, Marie-Luise
AU  - Schneider, Anja
AU  - Schneider, Nina
AU  - Coraggio, Francesca
AU  - Tolkachov, Alexander
AU  - Nguyen, Xuan Phuoc
AU  - Vilkaite, Adriana
AU  - Rehnitz, Julia
AU  - Odom, Duncan
AU  - Goncalves, Angela
TI  - Granulosa cell transcription is similarly impacted by superovulation and aging and predicts early embryonic trajectories.
JO  - Nature Communications
VL  - 16
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Springer Nature
M1  - DKFZ-2025-00839
SP  - 3658
PY  - 2025
N1  - #EA:B270#EA:C220#LA:B270#
AB  - In vitro fertilization efficiency is limited in part because a fraction of retrieved oocytes fails to fertilize. Accurately evaluating their quality could significantly improve in vitro fertilization efficiency, which would require better understanding how their maturation may be disrupted. Here, we quantitatively investigate the interplay between superovulation and aging in mouse oocytes and their paired granulosa cells using a newly adapted experimental methodology. We test the hypothesis that superovulation disrupts oocyte maturation, revealing the key intercellular communication pathways dysregulated at the transcriptional level by forced hormonal stimulation. We further demonstrate that granulosa cell transcriptional markers can prospectively predict an associated oocyte's early developmental potential. By using naturally ovulated old mice as a non-stimulated reference, we show that aging and superovulation dysregulate similar genes and interact with each other. By comparing mice and human transcriptional responses of granulosa cells, we find that age-related dysregulation of hormonal responses and cell cycle pathways are shared, though substantial divergence exists in other pathways.
KW  - Female
KW  - Granulosa Cells: metabolism
KW  - Granulosa Cells: cytology
KW  - Animals
KW  - Superovulation: genetics
KW  - Superovulation: physiology
KW  - Aging: genetics
KW  - Aging: physiology
KW  - Mice
KW  - Humans
KW  - Oocytes: metabolism
KW  - Oocytes: cytology
KW  - Oocytes: physiology
KW  - Transcription, Genetic
KW  - Mice, Inbred C57BL
KW  - Fertilization in Vitro
KW  - Embryonic Development: genetics
KW  - Gene Expression Regulation, Developmental
LB  - PUB:(DE-HGF)16
C6  - pmid:40246835
C2  - pmc:PMC12006393
DO  - DOI:10.1038/s41467-025-58451-9
UR  - https://inrepo02.dkfz.de/record/300625
ER  -