000300702 001__ 300702 000300702 005__ 20250504020334.0 000300702 0247_ $$2doi$$a10.1038/s41418-025-01517-4 000300702 0247_ $$2pmid$$apmid:40281125 000300702 0247_ $$2ISSN$$a1350-9047 000300702 0247_ $$2ISSN$$a1476-5403 000300702 0247_ $$2altmetric$$aaltmetric:176467914 000300702 037__ $$aDKFZ-2025-00882 000300702 041__ $$aEnglish 000300702 082__ $$a610 000300702 1001_ $$00009-0004-9298-8315$$aYapici, F Isil$$b0 000300702 245__ $$aAn atlas of ferroptosis-induced secretomes. 000300702 260__ $$a[London]$$bSpringer Nature$$c2025 000300702 3367_ $$2DRIVER$$aarticle 000300702 3367_ $$2DataCite$$aOutput Types/Journal article 000300702 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1745840955_34 000300702 3367_ $$2BibTeX$$aARTICLE 000300702 3367_ $$2ORCID$$aJOURNAL_ARTICLE 000300702 3367_ $$00$$2EndNote$$aJournal Article 000300702 500__ $$aepub / DKFZ-ZMBH Alliance 000300702 520__ $$aCells undergoing regulated necrosis systemically communicate with the immune system via the release of protein and non-protein secretomes. Ferroptosis is a recently described iron-dependent type of regulated necrosis driven by massive lipid peroxidation. While membrane rupture occurs during ferroptosis, a comprehensive appraisal of ferroptotic secretomes and their potential biological activity has been lacking. Here, we apply a multi-omics approach to provide an atlas of ferroptosis-induced secretomes and reveal a novel function in macrophage priming. Proteins with assigned DAMP and innate immune system function, such as MIF, heat shock proteins (HSPs), and chaperones, were released from ferroptotic cells. Non-protein secretomes with assigned inflammatory function contained oxylipins as well as TCA- and methionine-cycle metabolites. Interestingly, incubation of bone marrow-derived macrophages (BMDMs) with ferroptotic supernatants induced transcriptional reprogramming consistent with priming. Indeed, exposure to ferroptotic supernatants enhanced LPS-induced cytokine production. These results define a catalog of ferroptosis-induced secretomes and identify a biological activity in macrophage priming with important implications for the fine-tuning of inflammatory processes. 000300702 536__ $$0G:(DE-HGF)POF4-311$$a311 - Zellbiologie und Tumorbiologie (POF4-311)$$cPOF4-311$$fPOF IV$$x0 000300702 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de 000300702 7001_ $$00000-0003-3334-3950$$aSeidel, Eric$$b1 000300702 7001_ $$00000-0001-9055-0695$$aDahlhaus, Alina$$b2 000300702 7001_ $$aWeber, Josephine$$b3 000300702 7001_ $$00000-0002-3867-0881$$aSchmidt, Christina$$b4 000300702 7001_ $$0P:(DE-He78)5c3fb2db99f4cb5ee64d78f2d458d032$$ade Britto Chaves Filho, Adriano$$b5$$udkfz 000300702 7001_ $$aYang, Ming$$b6 000300702 7001_ $$00009-0008-2522-9641$$aNenchova, Maria$$b7 000300702 7001_ $$00009-0008-7244-1469$$aGüngör, Emre$$b8 000300702 7001_ $$aStroh, Jenny$$b9 000300702 7001_ $$aKotouza, Ioanna$$b10 000300702 7001_ $$aBeck, Julia$$b11 000300702 7001_ $$aAbdallah, Ali T$$b12 000300702 7001_ $$00000-0001-8182-8034$$aLackmann, Jan-Wilm$$b13 000300702 7001_ $$00000-0002-7841-7493$$aBebber, Christina M$$b14 000300702 7001_ $$aAndroulidaki, Ariadne$$b15 000300702 7001_ $$00000-0001-8684-9424$$aKreuzaler, Peter$$b16 000300702 7001_ $$0P:(DE-He78)94ae391f53fb9285e1b68f9930615af1$$aSchulze, Almut$$b17$$udkfz 000300702 7001_ $$aFrezza, Christian$$b18 000300702 7001_ $$00000-0002-7816-5919$$avon Karstedt, Silvia$$b19 000300702 773__ $$0PERI:(DE-600)1496681-5$$a10.1038/s41418-025-01517-4$$pnn$$tCell death and differentiation$$vnn$$x1350-9047$$y2025 000300702 909CO $$ooai:inrepo02.dkfz.de:300702$$pVDB 000300702 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)5c3fb2db99f4cb5ee64d78f2d458d032$$aDeutsches Krebsforschungszentrum$$b5$$kDKFZ 000300702 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)94ae391f53fb9285e1b68f9930615af1$$aDeutsches Krebsforschungszentrum$$b17$$kDKFZ 000300702 9131_ $$0G:(DE-HGF)POF4-311$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vZellbiologie und Tumorbiologie$$x0 000300702 9141_ $$y2025 000300702 915__ $$0StatID:(DE-HGF)3002$$2StatID$$aDEAL Springer$$d2024-12-28$$wger 000300702 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bCELL DEATH DIFFER : 2022$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-28 000300702 915__ $$0StatID:(DE-HGF)9910$$2StatID$$aIF >= 10$$bCELL DEATH DIFFER : 2022$$d2024-12-28 000300702 9201_ $$0I:(DE-He78)A410-20160331$$kA410$$lMetabolismus und Microenvironment$$x0 000300702 980__ $$ajournal 000300702 980__ $$aVDB 000300702 980__ $$aI:(DE-He78)A410-20160331 000300702 980__ $$aUNRESTRICTED