% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Bodensohn:300744,
      author       = {U. S. Bodensohn and B. Dünschede and C. Kuhlmann and K.
                      Kumari and R. Ladig$^*$ and C. Grefen and E. Schleiff and D.
                      Fernandez and D. Schünemann},
      title        = {{GET}3{B} is involved in chloroplast biogenesis and
                      interacts with the thylakoidal {ALB}3 and {ALB}4
                      insertases.},
      journal      = {Plant cell reports},
      volume       = {44},
      number       = {5},
      issn         = {0721-7714},
      address      = {Heidelberg [u.a.]},
      publisher    = {Springer},
      reportid     = {DKFZ-2025-00904},
      pages        = {108},
      year         = {2025},
      abstract     = {Proteomic, functional physiological analyses of get3b
                      mutant plants highlight GET3B's role in chloroplast
                      function. Genetic and interaction analyses indicate get3b
                      and srp54 as mutual potentiators that might share terminal
                      insertases. Protein targeting and insertion into membranes
                      are essential for cellular organization and organelle
                      function. The Guided Entry of Tail-anchored (GET) pathway
                      facilitates the post-translational targeting and insertion
                      of tail-anchored (TA) membrane proteins. Arabidopsis
                      thaliana has four GET3 homologues, including AtGET3B and
                      AtGET3D localized to chloroplasts. These photosynthetic
                      organelles possess complex membrane systems, and the
                      mechanisms underlying their protein targeting and membrane
                      biogenesis are not fully understood. This study conducted a
                      comprehensive proteomic analysis of get3b mutant plastids,
                      which displayed significant alterations. Fluorometric based
                      complex assembly as well as CO2 assimilation analyses
                      confirmed that disruption of GET3B function displayed a
                      significant impact on photosystem II assembly as well as
                      carbon fixation, respectively, indicating a functional role
                      in chloroplast biogenesis. Additionally, genetic
                      interactions were found between GET3B and the two component
                      STIC system, which cooperates with the cpSRP pathway which
                      is involved in the co-translational sorting of thylakoid
                      proteins. Further, physical interactions were observed
                      between GET3B and the C-terminus of ALB3 and ALB4 in vitro
                      and the full length proteins in vivo, indicating a role of
                      GET3B in protein targeting and membrane integration within
                      chloroplasts. These findings enhance our understanding of
                      GET3B's involvement in stromal protein targeting and
                      thylakoidal biogenesis.},
      keywords     = {Arabidopsis Proteins: metabolism / Arabidopsis Proteins:
                      genetics / Arabidopsis: metabolism / Arabidopsis: genetics /
                      Chloroplasts: metabolism / Thylakoids: metabolism /
                      Photosystem II Protein Complex: metabolism / Mutation / ALB3
                      (Other) / ALB4 (Other) / GET3B (Other) / STIC1 (Other) /
                      STIC2 (Other) / cpSRP54 (Other) / Arabidopsis Proteins (NLM
                      Chemicals) / ALBINO 3 protein, Arabidopsis (NLM Chemicals) /
                      Photosystem II Protein Complex (NLM Chemicals)},
      cin          = {B230},
      ddc          = {580},
      cid          = {I:(DE-He78)B230-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40299103},
      pmc          = {pmc:PMC12040988},
      doi          = {10.1007/s00299-025-03500-2},
      url          = {https://inrepo02.dkfz.de/record/300744},
}