TY  - JOUR
AU  - De Cock, Lore
AU  - Palubeckaitė, Ieva
AU  - Bersani, Francesca
AU  - Faehling, Tobias
AU  - Pasquali, Sandro
AU  - Umbaugh, Sam
AU  - Meister, Michael Torsten
AU  - Danks, Molly R
AU  - Manasterski, Piotr
AU  - Miallot, Richard
AU  - Krumbholz, Manuela
AU  - Roohani, Siyer
AU  - Heymann, Dominique
AU  - Cidre-Aranaz, Florencia
AU  - Wozniak, Agnieszka
AU  - Schöffski, Patrick
AU  - Bovée, Judith V M G
AU  - Merlini, Alessandra
AU  - Venneker, Sanne
TI  - Establishment of patient-derived 3D in vitro models of sarcomas: literature review and guidelines on behalf of the FORTRESS working group.
JO  - Neoplasia
VL  - 65
SN  - 1522-8002
CY  - Basingstoke
PB  - Stockton Press
M1  - DKFZ-2025-00925
SP  - 101171
PY  - 2025
AB  - Sarcomas are a large family of rare and heterogeneous mesenchymal tumors, which respond poorly to available systemic treatments. Translation of preclinical findings into clinical applications has been slow, limiting improvements in patients' outcomes and ultimately highlighting the need for a better understanding of sarcoma biology to develop more effective, subtype-specific therapies. To this end, reliable preclinical models are crucial, but the development of 3D in vitro sarcoma models has been lagging behind that of epithelial cancers. This is primarily due to the rarity and heterogeneity of sarcomas, and lack of widespread knowledge regarding the optimal growth conditions of these in vitro models. In this review, we provide an overview of currently available sarcoma tumoroid models, together with guidelines and suggestions for model development and characterization, on behalf of the FORTRESS (Forum For Translational Research in Sarcomas) international research working group on 3D sarcoma models.
KW  - 3D in vitro models (Other)
KW  - Patient-derived preclinical models (Other)
KW  - Sarcoma (Other)
KW  - Tumoroids (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40324303
DO  - DOI:10.1016/j.neo.2025.101171
UR  - https://inrepo02.dkfz.de/record/300805
ER  -