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@ARTICLE{Schwalm:301267,
      author       = {M. P. Schwalm$^*$ and C. Lenz and K. Saxena$^*$ and D. J.
                      Klionsky and E. Proschak and S. Knapp$^*$},
      title        = {{B}iochemical investigation of {LC}3/{GABARAP}-ligand
                      interaction as an important quality measure for
                      {LC}3/{GABARAP}-targeting small molecules: addendum to the
                      guidelines (4th edition).},
      journal      = {Autophagy},
      volume       = {21},
      number       = {9},
      issn         = {1554-8627},
      address      = {Abingdon, Oxon},
      publisher    = {Taylor $\&$ Francis},
      reportid     = {DKFZ-2025-00952},
      pages        = {2069-2073},
      year         = {2025},
      note         = {ISSN 1554-8635 / 2025 Sep;21(9):2069-2073},
      abstract     = {Targeted protein degradation (TPD) represents a new
                      therapeutic modality that allows the targeting of proteins
                      that are considered undruggable by conventional small
                      molecules. While TPD approaches via the ubiquitin-proteasome
                      system are well established and validated, additional
                      degradation pathways still require rigorous
                      characterization. Here, we focus on macroautophagy/autophagy
                      tethering compounds, a class of small molecules, designed to
                      recruit cargo to LC3/GABARAP proteins for subsequent
                      autophagosome-dependent degradation. We provide guidance for
                      the biophysical and structural characterization of small
                      molecule modulators for studying LC3/GABARAP-ligand
                      interactions. In addition, we discuss potential limitations
                      of autophagy-based TPD systems and emphasize the need for
                      rigorous quality control in the development of
                      LC3/GABARAP-targeting small molecules.Abbreviations: DSF:
                      differential scanning fluorimetry; FP: fluorescence
                      polarization; FRET: Förster/fluorescence resonance energy
                      transfer; HTRF: homogeneous time-resolved fluorescence; ITC:
                      isothermal titration calorimetry; LIR: LC3-interacting
                      region; MGs: molecular glues; NMR: nuclear magnetic
                      resonance; PROTACs: PROteolysis-TArgeting Chimeras; SPR:
                      surface plasmon resonance; TPD: targeted protein
                      degradation; TR-FRET: time-resolved Förster/fluorescence
                      resonance energy transfer; UPS: ubiquitin-proteasome
                      system.},
      keywords     = {ATTECs (Other) / AUTACs (Other) / Atg8 (Other) / GABARAP
                      (Other) / LC3 (Other)},
      cin          = {FM01},
      ddc          = {570},
      cid          = {I:(DE-He78)FM01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40344429},
      doi          = {10.1080/15548627.2025.2498506},
      url          = {https://inrepo02.dkfz.de/record/301267},
}