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@ARTICLE{Ahmad:301369,
author = {S. T. Ahmad and Y. Li and J. Garcia-Lopez and B. L. Gudenas
and J. Hadley and L. Paul and S. C. Wu and A. Refaat and M.
Kojic and M. Batts and T. Soliman and A. Pitre and F.
Arnskötter$^*$ and F. Zindy and A. Jones and N. R. Twarog
and A. Mayasundari and B. Bianski and C. Tinkle and A.
Shirinifard and L. Janke and M. Lu and S. A. Lewis and A.
Onar-Thomas and S. M. Pfister$^*$ and A. Gajjar and S. J.
Baker and M. F. Roussel and Z. Rankovic and G. W. Robinson
and B. A. Orr and B. Wainwright and A. A. Shelat and S. M.
Waszak and L. M. Kutscher$^*$ and H. Lin and P. A.
Northcott},
title = {{G}enetic modeling of {ELP}1-associated {S}onic hedgehog
medulloblastoma identifies {MDM}2 as a selective therapeutic
target.},
journal = {Cancer cell},
volume = {43},
number = {6},
issn = {1535-6108},
address = {Cambridge, Mass.},
publisher = {Cell Press},
reportid = {DKFZ-2025-01007},
pages = {1141-1158.e11},
year = {2025},
note = {2025 Jun 9;43(6):1141-1158.e11},
abstract = {Germline loss-of-function (LOF) variants in Elongator
acetyltransferase complex subunit 1 (ELP1) are the most
prevalent predisposing genetic events in childhood
medulloblastoma (MB), accounting for $∼30\%$ of the Sonic
hedgehog (SHH) 3 subtype. The mechanism(s) by which germline
ELP1 deficiency provokes SHH-MB pathogenesis remain unknown.
Genetically engineered mice mimicking heterozygous Elp1 LOF
(Elp1HET) seen in affected germline carriers exhibit
hallmark features of premalignancy in cerebellar granule
neuron progenitors (GNPs), including increased DNA
replication stress, genomic instability, accelerated cell
cycle, and stalled differentiation. Orthotopic
transplantation of Elp1HET GNPs harboring somatic Ptch1
inactivation yields SHH-MB-like tumors with compromised p53
signaling, providing a plausible explanation for the
exclusivity of ELP1-associated MBs in the SHH-3 subtype.
Preclinical treatment of ELP1-mutant patient-derived
xenografts with an FDA-approved MDM2 inhibitor reactivates
p53-dependent apoptosis and extends survival. Our findings
functionally substantiate the role of ELP1 deficiency in
SHH-MB predisposition and nominate therapeutics targeting
MDM2 as a rational treatment option.},
keywords = {ELP1 (Other) / Elongator complex (Other) / Sonic hedgehog
signaling (Other) / cerebellar development (Other) /
childhood cancer predisposition (Other) / genetically
engineered mouse models (Other) / granule neuron progenitors
(Other) / in vivo preclinical studies (Other) /
medulloblastoma (Other) / multi-omics (Other)},
cin = {B430 / B062 / HD01},
ddc = {610},
cid = {I:(DE-He78)B430-20160331 / I:(DE-He78)B062-20160331 /
I:(DE-He78)HD01-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40378836},
doi = {10.1016/j.ccell.2025.04.014},
url = {https://inrepo02.dkfz.de/record/301369},
}