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000301494 1001_ $$0P:(DE-HGF)0$$aAviles-Huerta, Daniela$$b0$$eFirst author
000301494 245__ $$aDual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironment.
000301494 260__ $$a[London]$$bSpringer Nature$$c2025
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000301494 520__ $$aThe tumor microenvironment (TME) influences cancer cell metabolism and survival. However, how immune and stromal cells respond to metabolic stress in vivo, and how nutrient limitations affect therapy, remains poorly understood. Here, we introduce Dual Ribosome Profiling (DualRP) to simultaneously monitor translation and ribosome stalling in multiple tumor cell populations. DualRP reveals that cancer-fibroblast interactions trigger an inflammatory program that reduces amino acid shortages during glucose starvation. In immunocompetent mice, we show that serine and glycine are essential for optimal T cell function and that their deficiency impairs T cell fitness. Importantly, immune checkpoint blockade therapy imposes amino acid restrictions specifically in T cells, demonstrating that therapies create distinct metabolic demands across TME cell types. By mapping codon-resolved ribosome stalling in a cell‑type‑specific manner, DualRP uncovers metabolic crosstalk that shapes translational programs. DualRP thus offers a powerful, innovative approach for dissecting tumor cell metabolic interplay and guiding combined metabolic-immunotherapeutic strategies.
000301494 536__ $$0G:(DE-HGF)POF4-312$$a312 - Funktionelle und strukturelle Genomforschung (POF4-312)$$cPOF4-312$$fPOF IV$$x0
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000301494 650_7 $$0TE7660XO1C$$2NLM Chemicals$$aGlycine
000301494 650_7 $$2NLM Chemicals$$aAmino Acids
000301494 650_7 $$0452VLY9402$$2NLM Chemicals$$aSerine
000301494 650_2 $$2MeSH$$aTumor Microenvironment: immunology
000301494 650_2 $$2MeSH$$aTumor Microenvironment: genetics
000301494 650_2 $$2MeSH$$aAnimals
000301494 650_2 $$2MeSH$$aRibosomes: metabolism
000301494 650_2 $$2MeSH$$aRibosomes: genetics
000301494 650_2 $$2MeSH$$aMice
000301494 650_2 $$2MeSH$$aHumans
000301494 650_2 $$2MeSH$$aStromal Cells: metabolism
000301494 650_2 $$2MeSH$$aNeoplasms: metabolism
000301494 650_2 $$2MeSH$$aNeoplasms: pathology
000301494 650_2 $$2MeSH$$aNeoplasms: genetics
000301494 650_2 $$2MeSH$$aNeoplasms: immunology
000301494 650_2 $$2MeSH$$aCell Line, Tumor
000301494 650_2 $$2MeSH$$aT-Lymphocytes: metabolism
000301494 650_2 $$2MeSH$$aT-Lymphocytes: immunology
000301494 650_2 $$2MeSH$$aGlycine: metabolism
000301494 650_2 $$2MeSH$$aProtein Biosynthesis
000301494 650_2 $$2MeSH$$aAmino Acids: metabolism
000301494 650_2 $$2MeSH$$aMice, Inbred C57BL
000301494 650_2 $$2MeSH$$aSerine: metabolism
000301494 650_2 $$2MeSH$$aFemale
000301494 650_2 $$2MeSH$$aFibroblasts: metabolism
000301494 650_2 $$2MeSH$$aRibosome Profiling
000301494 7001_ $$0P:(DE-He78)5944c192df14d8441fa71121b91b96d9$$aDel Pizzo, Rossella$$b1$$eFirst author$$udkfz
000301494 7001_ $$0P:(DE-He78)de7b14bf7f622d35c8dabed808304bc9$$aKowar, Alexander$$b2$$udkfz
000301494 7001_ $$0P:(DE-He78)9bae5e495cd17da99206bde0a310a87e$$aBaig, Ali Hyder$$b3
000301494 7001_ $$0P:(DE-He78)739e39176c0b4e701c9ae425485c2912$$aPalazzo, Giuliana$$b4$$udkfz
000301494 7001_ $$0P:(DE-He78)dc4f734225935c3650fde7619c82ed92$$aStepanova, Ekaterina$$b5
000301494 7001_ $$0P:(DE-He78)1f71ccf4137962d5dbf12200b9ffa75d$$aAmaya Ramirez, Cinthia Claudia$$b6
000301494 7001_ $$0P:(DE-He78)e3b2ae3dbeb65ca1ccba552e063133a7$$aD'Agostino, Sara$$b7
000301494 7001_ $$0P:(DE-He78)319f5ff79bf5e1c826c5c2ae2c2c9a15$$aRatto, Edoardo$$b8
000301494 7001_ $$00000-0002-4650-4250$$aPechincha, Catarina$$b9
000301494 7001_ $$0P:(DE-He78)25f23768b98d2258fdb394f856542ba7$$aSiefert, Nora Sophie$$b10$$udkfz
000301494 7001_ $$0P:(DE-He78)1a89b3d8ddc335a4c010ef9d5efabc7a$$aEngel, Helena$$b11$$udkfz
000301494 7001_ $$0P:(DE-He78)842f32f9066384840481c90552ab2cff$$aDu, Shangce$$b12$$udkfz
000301494 7001_ $$aCadenas-De Miguel, Silvia$$b13
000301494 7001_ $$0P:(DE-He78)b5b786a7a28d851956ba90aa9451887a$$aMiao, Beiping$$b14$$udkfz
000301494 7001_ $$00009-0008-5269-4987$$aCruz-Vilchez, Victor M$$b15
000301494 7001_ $$0P:(DE-He78)799d978330dff449f8244947929a4518$$aMüller-Decker, Karin$$b16
000301494 7001_ $$00000-0003-0922-9429$$aElia, Ilaria$$b17
000301494 7001_ $$0P:(DE-He78)ef40d75e5564c492ee57b4262fc016fe$$aSun, Chong$$b18$$udkfz
000301494 7001_ $$0P:(DE-He78)c8525dbb77cddc5280375ea4a5e3c13e$$aPalm, Wilhelm$$b19$$udkfz
000301494 7001_ $$0P:(DE-He78)4af0380446b05b5f1995502016151a1b$$aLoayza-Puch, Fabricio$$b20$$eLast author$$udkfz
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