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@ARTICLE{AvilesHuerta:301494,
      author       = {D. Aviles-Huerta$^*$ and R. Del Pizzo$^*$ and A. Kowar$^*$
                      and A. H. Baig$^*$ and G. Palazzo$^*$ and E. Stepanova$^*$
                      and C. C. Amaya Ramirez$^*$ and S. D'Agostino$^*$ and E.
                      Ratto$^*$ and C. Pechincha$^*$ and N. S. Siefert$^*$ and H.
                      Engel$^*$ and S. Du$^*$ and S. Cadenas-De Miguel and B.
                      Miao$^*$ and V. M. Cruz-Vilchez$^*$ and K.
                      Müller-Decker$^*$ and I. Elia and C. Sun$^*$ and W.
                      Palm$^*$ and F. Loayza-Puch$^*$},
      title        = {{D}ual {R}ibosome {P}rofiling reveals metabolic limitations
                      of cancer and stromal cells in the tumor microenvironment.},
      journal      = {Nature Communications},
      volume       = {16},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2025-01036},
      pages        = {4652},
      year         = {2025},
      note         = {#EA:B250#LA:B250#},
      abstract     = {The tumor microenvironment (TME) influences cancer cell
                      metabolism and survival. However, how immune and stromal
                      cells respond to metabolic stress in vivo, and how nutrient
                      limitations affect therapy, remains poorly understood. Here,
                      we introduce Dual Ribosome Profiling (DualRP) to
                      simultaneously monitor translation and ribosome stalling in
                      multiple tumor cell populations. DualRP reveals that
                      cancer-fibroblast interactions trigger an inflammatory
                      program that reduces amino acid shortages during glucose
                      starvation. In immunocompetent mice, we show that serine and
                      glycine are essential for optimal T cell function and that
                      their deficiency impairs T cell fitness. Importantly, immune
                      checkpoint blockade therapy imposes amino acid restrictions
                      specifically in T cells, demonstrating that therapies create
                      distinct metabolic demands across TME cell types. By mapping
                      codon-resolved ribosome stalling in a cell‑type‑specific
                      manner, DualRP uncovers metabolic crosstalk that shapes
                      translational programs. DualRP thus offers a powerful,
                      innovative approach for dissecting tumor cell metabolic
                      interplay and guiding combined metabolic-immunotherapeutic
                      strategies.},
      keywords     = {Tumor Microenvironment: immunology / Tumor
                      Microenvironment: genetics / Animals / Ribosomes: metabolism
                      / Ribosomes: genetics / Mice / Humans / Stromal Cells:
                      metabolism / Neoplasms: metabolism / Neoplasms: pathology /
                      Neoplasms: genetics / Neoplasms: immunology / Cell Line,
                      Tumor / T-Lymphocytes: metabolism / T-Lymphocytes:
                      immunology / Glycine: metabolism / Protein Biosynthesis /
                      Amino Acids: metabolism / Mice, Inbred C57BL / Serine:
                      metabolism / Female / Fibroblasts: metabolism / Ribosome
                      Profiling / Glycine (NLM Chemicals) / Amino Acids (NLM
                      Chemicals) / Serine (NLM Chemicals)},
      cin          = {B250 / A330 / D250 / W420},
      ddc          = {500},
      cid          = {I:(DE-He78)B250-20160331 / I:(DE-He78)A330-20160331 /
                      I:(DE-He78)D250-20160331 / I:(DE-He78)W420-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40389477},
      doi          = {10.1038/s41467-025-59986-7},
      url          = {https://inrepo02.dkfz.de/record/301494},
}