% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kovacs:301913,
      author       = {D. Kovacs and K. Heger and P. Giansanti and C. Iuliano and
                      F. Meissner and M. Mann and J. Böttcher and R. Öllinger
                      and R. Rad$^*$ and F. Tammer and V. Gölling and T. Zeng and
                      A. Masjedi and T. G. Dr Med Vet and A. Roers and M. Babina
                      and M. S. Robles and M. Moser and S. Kaesler and K. S. Dr
                      Med Vet and T. Biedermann and M. Schmidt-Supprian$^*$},
      title        = {{M}ast cells modulate macrophage biology through release of
                      pre-stored {CSF}1.},
      journal      = {The journal of allergy and clinical immunology},
      volume       = {nn},
      issn         = {0091-6749},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2025-01183},
      pages        = {nn},
      year         = {2025},
      note         = {epub},
      abstract     = {Mast cells are tissue-resident immune cells present in
                      connective tissues throughout the body. They exert diverse
                      functions in immunity by rapidly releasing a plethora of
                      preformed mediators, including proteoglycans, cytokines, and
                      proteases, which are stored in cytoplasmic granules.Our aim
                      was to systematically and globally identify mast
                      cell-released protein mediators and elucidate their
                      functions.We analysed the secretomes of antigen-activated
                      primary mouse mast cells using quantitative mass
                      spectrometry-based proteomics and conducted follow-up
                      studies in vitro, ex vivo and using mast cell-specific
                      genetic mouse models.We identified CSF1 as a novel preformed
                      mast cell mediator present in the granules of all connective
                      tissue-type mast cells. We further show that the mast cell
                      secretome can induce macrophage differentiation and a unique
                      polarisation pattern via CSF1 and other mediators. Mast
                      cell-derived CSF1 has systemic functions, as mast
                      cell-specific CSF1-deficient mice have lower serum CSF1
                      levels and reduced numbers of circulating monocytes. In
                      addition, using an orthotopic transplantation-based melanoma
                      mouse model, we show that loss of mast cell-derived CSF1
                      promotes cancer cell expansion. Finally, we demonstrate that
                      CSF1 is also prestored and released by human mast cells.CSF1
                      is an evolutionarily conserved, constitutive mast cell
                      granule component. Mast cell degranulation induces
                      macrophage differentiation and a unique polarisation state,
                      the former being completely dependent on CSF1, while the
                      latter is only modulated.},
      keywords     = {CSF1 (Other) / mast cell (Other) / mast cell mediator
                      (Other) / melanoma (Other) / proteome (Other)},
      cin          = {MU01},
      ddc          = {610},
      cid          = {I:(DE-He78)MU01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40480612},
      doi          = {10.1016/j.jaci.2025.05.022},
      url          = {https://inrepo02.dkfz.de/record/301913},
}