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@ARTICLE{Kriukova:302005,
      author       = {E. Kriukova and M. Mazurenka and S. Marcazzan and M.
                      Tschurtschenthaler$^*$ and G. Puppels and S. Glasl and D.
                      Saur$^*$ and M. Jesinghaus and M. Pouliou and M. Agelopoulos
                      and A. Klinakis and M. Quante and J. Ripoll and V.
                      Ntziachristos and D. Gorpas},
      title        = {{P}robing {F}ield {C}ancerization in the {G}astrointestinal
                      {T}ract {U}sing a {H}ybrid {R}aman and {P}artial {W}ave
                      {S}pectroscopy {M}icroscope.},
      journal      = {Analytical chemistry},
      volume       = {97},
      number       = {24},
      issn         = {0003-2700},
      address      = {Columbus, Ohio},
      publisher    = {American Chemical Society},
      reportid     = {DKFZ-2025-01204},
      pages        = {12642-12653},
      year         = {2025},
      note         = {2025 Jun 24;97(24):12642-12653},
      abstract     = {Field cancerization (FC) refers to spatially distributed
                      premalignant tissue changes that lead to the appearance of
                      local malignancy, and its detection can improve cancer
                      screening. In this work, we employ combined Raman and
                      partial wave spectroscopy (RS-PWS) to detect FC in
                      gastroesophageal (L2-IL1B) and intestinal (Villin-Cre,
                      Apcfl/wt) tumor mouse models. Using a hybrid RS-PWS
                      microscope, we acquire both molecular and morphological
                      information from macroscopically normal tumor-adjacent
                      tissue and investigate the individual and combined
                      performance of each modality. For data analysis, we use
                      partial least-squares discriminant analysis (PLS-DA). In the
                      normal tissue of L2-IL1B mice, we demonstrate a
                      statistically significant increase (p < 0.001) in Raman band
                      intensities associated with free amino acids and a decrease
                      in bands associated with lipids (p < 0.005) and carotenoids
                      (p < 0.001) compared to healthy controls. Similarly, in the
                      normal mucosa of Villin-Cre, Apcfl/wt mice, the intensities
                      of RS bands associated with amino acids increase
                      significantly (p < 0.05) compared to controls, while the
                      intensities of lipid-associated bands decrease significantly
                      (p < 0.05). Transcriptomic profiling using RNA-sequencing
                      analysis on these samples identified a significant
                      correlation between gene expression and optical findings.
                      Moreover, we demonstrate that combining RS and PWS data
                      further improves the significance of our classification
                      results. When macroscopically normal tumor-adjacent tissue
                      is compared with tissue from healthy controls, we observe
                      that PWS increases the R2 of RS results by $∼9\%$ in
                      L2-IL1B mice and $∼5\%$ in Villin-Cre, Apcfl/wt mice.
                      Combining molecular RS with structural PWS information
                      enhances the ability to detect precancerous changes and
                      provides insights into tissue alterations during cancer
                      development.},
      cin          = {MU01},
      ddc          = {540},
      cid          = {I:(DE-He78)MU01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40497475},
      doi          = {10.1021/acs.analchem.5c00954},
      url          = {https://inrepo02.dkfz.de/record/302005},
}