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| Home > Publications database > Generation and functional analysis of melanoma antigen-specific CD8+ T cells derived from S/MAR vector-transfected human induced pluripotent stem cells. > print |
| Home > Publications database > Generation and functional analysis of melanoma antigen-specific CD8+ T cells derived from S/MAR vector-transfected human induced pluripotent stem cells. > print |
| 001 | 302016 | ||
| 005 | 20250904143553.0 | ||
| 024 | 7 | _ | |a 10.1002/ijc.35524 |2 doi |
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| 100 | 1 | _ | |a Poelchen, Juliane |0 P:(DE-He78)88a268b9173c651561385cddfdf819fa |b 0 |e First author |
| 245 | _ | _ | |a Generation and functional analysis of melanoma antigen-specific CD8+ T cells derived from S/MAR vector-transfected human induced pluripotent stem cells. |
| 260 | _ | _ | |a Bognor Regis |c 2025 |b Wiley-Liss |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1756989311_27125 |2 PUB:(DE-HGF) |
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| 500 | _ | _ | |a #EA:A370#LA:A370# / 2025 Nov 1;157(9):1876-1887 |
| 520 | _ | _ | |a Melanoma accounts for the majority of all skin cancer-related deaths with rising incidence rates. Adoptive cell therapies (ACT) with tumor antigen-specific CD8+ T cells derived from human-induced pluripotent stem cells (hiPSCs) might offer a promising treatment strategy for advanced malignant melanoma patients. In this study, we investigated two strategies for the generation of CD8+ T cells from hiPSCs expressing a T cell receptor (TCR) specific for the melanoma-associated antigen recognized by T cells (MART-1) or a chimeric antigen receptor (CAR) specific for the melanoma-associated chondroitin sulfate proteoglycan (MCSP), respectively. While the long-term co-culture of bioengineered OP9 stromal cells with CD34+ hematopoietic stem/progenitor cells (HSPCs) facilitated the generation of CD4 + CD8+ double-positive (DP) T cells, we encountered difficulties in obtaining high percentages of CD8+ single-positive (SP) T cells using this method. However, the replacement of the OP9 cells with a T cell differentiation kit enabled the generation of CD8+ SP T cells after 47 days. Despite a low expression of the T cell marker CD3 on the surface of the generated CD8+ SP T cells, we detected intracellular IFN-γ and surface CD107a expression upon stimulation. Moreover, the generated CD8+ SP T cells exhibited cytotoxic effects when co-cultured with melanoma cell lines. The use of scaffold/matrix attachment region (S/MAR) DNA vectors ensured persistent expression of the TCR or the CAR during differentiation of T cells. Hence, these findings demonstrate the potential as well as the challenges associated with using S/MAR DNA vector-transfected hiPSCs for the generation of melanoma antigen-specific CD8+ T cells. |
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| 650 | _ | 7 | |a T cells |2 Other |
| 650 | _ | 7 | |a adoptive cell therapy |2 Other |
| 650 | _ | 7 | |a hiPSCs. S/MAR vector |2 Other |
| 650 | _ | 7 | |a melanoma |2 Other |
| 700 | 1 | _ | |a Pardo, Sandra |0 P:(DE-HGF)0 |b 1 |
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| 700 | 1 | _ | |a Steinfass, Tamara |0 P:(DE-He78)a65fa5fbdeff5a1bb6ee7de07b820488 |b 4 |
| 700 | 1 | _ | |a Vierthaler, Marlene |0 P:(DE-He78)61de47f47323d5d0b1700e8213e5179f |b 5 |
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| 700 | 1 | _ | |a Wang, Yiman |0 P:(DE-He78)ada7e359a1f7451130dbc79595227bec |b 8 |
| 700 | 1 | _ | |a Nicolay, Jan Peter |0 P:(DE-He78)7b847bc226706f4fc5a573f1483c49d9 |b 9 |u dkfz |
| 700 | 1 | _ | |a Guermonprez, Pierre |b 10 |
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| 700 | 1 | _ | |a Utikal, Jochen |0 P:(DE-He78)a229f7724466e7efadf4a1ace1ff8af3 |b 13 |e Last author |u dkfz |
| 773 | _ | _ | |a 10.1002/ijc.35524 |g p. ijc.35524 |0 PERI:(DE-600)1474822-8 |n 9 |p 1876-1887 |t International journal of cancer |v 157 |y 2025 |x 0020-7136 |
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