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@ARTICLE{Seib:302283,
      author       = {J. Seib$^*$ and D. Höfler$^*$ and L. Hornetz$^*$ and N.
                      Ohl$^*$ and K. Götz$^*$ and K. Vogel$^*$ and J. Butt$^*$
                      and K. Schmidt$^*$},
      title        = {{M}urine astrovirus infection course and antibody response
                      in different mouse strains.},
      journal      = {Lab animal},
      volume       = {54},
      issn         = {0093-7355},
      address      = {New York, NY},
      publisher    = {Nature Publ. Co.},
      reportid     = {DKFZ-2025-01302},
      pages        = {178–187},
      year         = {2025},
      note         = {#EA:W440#LA:W440# / 54, pages 178–187 (2025)},
      abstract     = {Monitoring the health of mice used in animal experiments
                      constitutes an important instrument toward microbiological
                      standardization, as infections can alter physiological
                      parameters and immune reactions and may therefore have an
                      essential impact on experimental outcome. In view of the
                      high prevalence rates of murine astrovirus (MuAstV)
                      infections in laboratory mouse facilities worldwide and the
                      potential impact on research outcomes and reproducibility,
                      there is a need to include MuAstV in the existing laboratory
                      mice health monitoring programs. Here, to determine the
                      sentinel strain and diagnostic method of choice, we aimed to
                      assess the course of MuAstV infection and the resulting
                      immune response in three immunocompetent mouse strains
                      (Crl:CD1 (ICR), C57BL/6J and BALB/cOlaHsd) and one
                      immunodeficient strain (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) by
                      analyzing blood, feces and tissue samples with
                      MuAstV-specific polymerase chain reaction and serology.
                      Depending on the mouse strain, the duration of infection and
                      viral load differed significantly, as well as the rapidity
                      and quantity of antibody production. Virus shedding in
                      immunocompetent mice was limited to a maximum of 4 weeks,
                      whereas immunodeficient mice shed virus for the entire
                      duration of this study. A fast antibody response with high
                      titers was found only in outbred CD1 mice. In C57BL/6J and
                      BALB/c mice, however, seropositivity and high antibody
                      levels were reached only after the second infection. These
                      results not only improve our understanding of the infection
                      characteristics of MuAstV, presumably the most prevalent
                      virus in laboratory mice, but also help to set up a health
                      monitoring routine giving meaningful and reliable results on
                      the MuAstV infection status of laboratory mouse
                      populations.},
      cin          = {W440 / D320 / W410},
      ddc          = {630},
      cid          = {I:(DE-He78)W440-20160331 / I:(DE-He78)D320-20160331 /
                      I:(DE-He78)W410-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40571749},
      doi          = {10.1038/s41684-025-01573-w},
      url          = {https://inrepo02.dkfz.de/record/302283},
}