% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Lattanzi:302798,
      author       = {C. Lattanzi and F. Bianchetto-Aguilera and M. Donini and F.
                      Pettinella and E. Caveggion and M. Castellucci and S.
                      Gasperini and B. Mariotti and I. Signoretto and M. Cantini
                      and S. Pilotto and L. Belluomini and C. Tecchio and F.
                      Bazzoni and S. Brandau$^*$ and N. Tamassia and M. A.
                      Cassatella and P. Scapini},
      title        = {{U}ncovering common transcriptional features shared by
                      mature peripheral blood {PMN}-{MDSC}s and tumor-infiltrating
                      neutrophils in humans.},
      journal      = {OncoImmunology},
      volume       = {14},
      number       = {1},
      issn         = {2162-4011},
      address      = {Abingdon},
      publisher    = {Taylor $\&$ Franics},
      reportid     = {DKFZ-2025-01338},
      pages        = {2521396},
      year         = {2025},
      abstract     = {Human polymorphonuclear-myeloid-derived suppressor cells
                      (PMN-MDSCs) consist of circulating low-density neutrophils
                      (LDNs) characterized by the ability to inhibit T-cell
                      responses. In previous studies, we demonstrated that the
                      mature fraction of PMN-MDSCs (i.e. mPMN-MDSCs) exerts more
                      potent immunosuppressive functions than its immature
                      counterpart. More recently, we defined a specific gene
                      signature of mPMN-MDSCs from cancer patients and
                      G-CSF-treated donors (GDs) by bulk RNA sequencing (RNA-seq)
                      experiments. In this study, by performing single-cell
                      RNA-seq (scRNA-seq) experiments of circulating mPMN-MDSCs
                      from non-small cell lung cancer (NSCLC) patients, we
                      identified a major scRNA-seq cell cluster (arbitrarily named
                      NSCLC c6) specifically displaying immunosuppressive and
                      protumor transcriptomic features. Then, by analyzing
                      publicly available scRNA-seq datasets from human
                      tumor-associated neutrophils (TANs), we uncovered three TAN
                      clusters (arbitrarily named TAN c6-c8) that were found to
                      share with NSCLC c6 several common genes and transcription
                      factor (TF) regulons associated with response to hypoxia,
                      positive regulation of angiogenesis, and metabolic
                      reprogramming. Furthermore, by performing scRNA-seq
                      experiments of GD mPMN-MDSCs, we uncovered four scRNA-seq
                      cell clusters (arbitrarily named GD c4-c7) that were
                      enriched for the same genes and pathways characterizing
                      NSCLC c6 and TAN c6-c8 cells. Altogether, these data uncover
                      that human circulating mPMN-MDSCs and TANs from different
                      cancer types share scRNA-seq cell clusters with
                      transcriptomic similarities, supporting the notion that they
                      might be strictly related.},
      keywords     = {Humans / Neutrophils: immunology / Neutrophils: metabolism
                      / Neutrophils: pathology / Carcinoma, Non-Small-Cell Lung:
                      genetics / Carcinoma, Non-Small-Cell Lung: immunology /
                      Carcinoma, Non-Small-Cell Lung: pathology / Lung Neoplasms:
                      genetics / Lung Neoplasms: immunology / Lung Neoplasms:
                      pathology / Myeloid-Derived Suppressor Cells: metabolism /
                      Myeloid-Derived Suppressor Cells: immunology /
                      Myeloid-Derived Suppressor Cells: pathology / Single-Cell
                      Analysis / Gene Expression Regulation, Neoplastic /
                      Transcriptome / Gene Expression Profiling / Tumor
                      Microenvironment: immunology / G-CSF (Other) / Neutrophils
                      (Other) / PMN-MDSCs (Other) / TANs (Other) / scRNA-seq
                      (Other)},
      cin          = {ED01},
      ddc          = {610},
      cid          = {I:(DE-He78)ED01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40590753},
      pmc          = {pmc:PMC12218443},
      doi          = {10.1080/2162402X.2025.2521396},
      url          = {https://inrepo02.dkfz.de/record/302798},
}