% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Sun:302830,
      author       = {D.-E. Sun and S. W. Ng$^*$ and Y. Zheng and S. Xie and N.
                      Schwan and P. Breuer and D. C. F. Hoffmann$^*$ and J. J.
                      Michel$^*$ and D. D. Azorin$^*$ and K. E. Boonekamp$^*$ and
                      F. Winkler$^*$ and W. Wick$^*$ and M. Boutros$^*$ and Y. Li
                      and K. Johnsson},
      title        = {{M}olecular recording of cellular protein kinase activity
                      with chemical labeling.},
      journal      = {Nature chemical biology},
      volume       = {nn},
      issn         = {1552-4450},
      address      = {Basingstoke},
      publisher    = {Nature Publishing Group},
      reportid     = {DKFZ-2025-01370},
      pages        = {nn},
      year         = {2025},
      note         = {epub},
      abstract     = {Protein kinases control most cellular processes and
                      aberrant kinase activity is involved in numerous diseases.
                      Here we introduce molecular recorders of kinase activities
                      for later analysis to investigate the link between specific
                      kinase activities and cellular phenotypes in heterogeneous
                      cell populations and in vivo. Based on split-HaloTag and a
                      phosphorylation-dependent molecular switch, our recorders
                      become rapidly labeled in the presence of a specific kinase
                      activity and a fluorescent HaloTag substrate. The kinase
                      activity in a given cell controls the degree of fluorescent
                      labeling, whereas the recording window is set by the
                      presence of the fluorescent substrate. We designed specific
                      recorders for four protein kinases, including protein kinase
                      A. We apply our protein kinase A recorder to sort
                      heterogeneous cell populations for subsequent transcriptome
                      analysis, in genome-wide CRISPR screens to discover
                      regulators of PKA activity and to track neuromodulation in
                      freely moving mice.},
      cin          = {B110 / B320 / HD01},
      ddc          = {570},
      cid          = {I:(DE-He78)B110-20160331 / I:(DE-He78)B320-20160331 /
                      I:(DE-He78)HD01-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40640555},
      doi          = {10.1038/s41589-025-01949-6},
      url          = {https://inrepo02.dkfz.de/record/302830},
}