guest ::
| Home > Publications database > Molecular recording of cellular protein kinase activity with chemical labeling. > print |
| Home > Publications database > Molecular recording of cellular protein kinase activity with chemical labeling. > print |
| 001 | 302830 | ||
| 005 | 20251030141932.0 | ||
| 024 | 7 | _ | |a 10.1038/s41589-025-01949-6 |2 doi |
| 024 | 7 | _ | |a pmid:40640555 |2 pmid |
| 024 | 7 | _ | |a 1552-4450 |2 ISSN |
| 024 | 7 | _ | |a 1552-4469 |2 ISSN |
| 024 | 7 | _ | |a altmetric:179231794 |2 altmetric |
| 037 | _ | _ | |a DKFZ-2025-01370 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Sun, De-En |b 0 |
| 245 | _ | _ | |a Molecular recording of cellular protein kinase activity with chemical labeling. |
| 260 | _ | _ | |a Basingstoke |c 2025 |b Nature Publishing Group |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1761830325_4133646 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a 2025 Nov;21(11):1818-1827 |
| 520 | _ | _ | |a Protein kinases control most cellular processes and aberrant kinase activity is involved in numerous diseases. Here we introduce molecular recorders of kinase activities for later analysis to investigate the link between specific kinase activities and cellular phenotypes in heterogeneous cell populations and in vivo. Based on split-HaloTag and a phosphorylation-dependent molecular switch, our recorders become rapidly labeled in the presence of a specific kinase activity and a fluorescent HaloTag substrate. The kinase activity in a given cell controls the degree of fluorescent labeling, whereas the recording window is set by the presence of the fluorescent substrate. We designed specific recorders for four protein kinases, including protein kinase A. We apply our protein kinase A recorder to sort heterogeneous cell populations for subsequent transcriptome analysis, in genome-wide CRISPR screens to discover regulators of PKA activity and to track neuromodulation in freely moving mice. |
| 536 | _ | _ | |a 312 - Funktionelle und strukturelle Genomforschung (POF4-312) |0 G:(DE-HGF)POF4-312 |c POF4-312 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de |
| 700 | 1 | _ | |a Ng, Siu Wang |0 P:(DE-He78)f27f7e1149101ac1b6818dfd03707fe6 |b 1 |u dkfz |
| 700 | 1 | _ | |a Zheng, Yu |b 2 |
| 700 | 1 | _ | |a Xie, Shu |b 3 |
| 700 | 1 | _ | |a Schwan, Niklas |0 0009-0002-1375-7590 |b 4 |
| 700 | 1 | _ | |a Breuer, Paula |0 0009-0005-6741-2303 |b 5 |
| 700 | 1 | _ | |a Hoffmann, Dirk Carsten Frieder |0 P:(DE-He78)e50ff195896914d35f54ed1bf913e270 |b 6 |u dkfz |
| 700 | 1 | _ | |a Michel, Julius Jakob |0 P:(DE-He78)513be409835c710d4fe381543a45b410 |b 7 |u dkfz |
| 700 | 1 | _ | |a Azorin, Daniel D |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Boonekamp, Kim Elisabeth |0 P:(DE-He78)a1fe2ba34f6963f2462d1c670b4c206d |b 9 |u dkfz |
| 700 | 1 | _ | |a Winkler, Frank |0 P:(DE-He78)6c294453ee36ad59deddc5494fa6aa4b |b 10 |u dkfz |
| 700 | 1 | _ | |a Wick, Wolfgang |0 P:(DE-He78)92e9783ca7025f36ce14e12cd348d2ee |b 11 |u dkfz |
| 700 | 1 | _ | |a Boutros, Michael |0 P:(DE-He78)3c0da8e3caa2aa50cad85152aa0465ad |b 12 |u dkfz |
| 700 | 1 | _ | |a Li, Yulong |0 0000-0002-9166-9919 |b 13 |
| 700 | 1 | _ | |a Johnsson, Kai |0 0000-0002-8002-1981 |b 14 |
| 773 | _ | _ | |a 10.1038/s41589-025-01949-6 |0 PERI:(DE-600)2190276-8 |n 11 |p 1818-1827 |t Nature chemical biology |v 21 |y 2025 |x 1552-4450 |
| 909 | C | O | |p VDB |o oai:inrepo02.dkfz.de:302830 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 1 |6 P:(DE-He78)f27f7e1149101ac1b6818dfd03707fe6 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 6 |6 P:(DE-He78)e50ff195896914d35f54ed1bf913e270 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 7 |6 P:(DE-He78)513be409835c710d4fe381543a45b410 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 8 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 9 |6 P:(DE-He78)a1fe2ba34f6963f2462d1c670b4c206d |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 10 |6 P:(DE-He78)6c294453ee36ad59deddc5494fa6aa4b |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 11 |6 P:(DE-He78)92e9783ca7025f36ce14e12cd348d2ee |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 12 |6 P:(DE-He78)3c0da8e3caa2aa50cad85152aa0465ad |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Krebsforschung |1 G:(DE-HGF)POF4-310 |0 G:(DE-HGF)POF4-312 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Funktionelle und strukturelle Genomforschung |x 0 |
| 914 | 1 | _ | |y 2025 |
| 915 | _ | _ | |a Nationallizenz |0 StatID:(DE-HGF)0420 |2 StatID |d 2024-12-20 |w ger |
| 915 | _ | _ | |a DEAL Nature |0 StatID:(DE-HGF)3003 |2 StatID |d 2024-12-20 |w ger |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1190 |2 StatID |b Biological Abstracts |d 2024-12-20 |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2024-12-20 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NAT CHEM BIOL : 2022 |d 2024-12-20 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search |d 2024-12-20 |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC |d 2024-12-20 |
| 915 | _ | _ | |a IF >= 10 |0 StatID:(DE-HGF)9910 |2 StatID |b NAT CHEM BIOL : 2022 |d 2024-12-20 |
| 920 | 1 | _ | |0 I:(DE-He78)B110-20160331 |k B110 |l B110 Signalwege funktionelle Genomik |x 0 |
| 920 | 1 | _ | |0 I:(DE-He78)B320-20160331 |k B320 |l KKE Neuroonkologie |x 1 |
| 920 | 1 | _ | |0 I:(DE-He78)HD01-20160331 |k HD01 |l DKTK HD zentral |x 2 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-He78)B110-20160331 |
| 980 | _ | _ | |a I:(DE-He78)B320-20160331 |
| 980 | _ | _ | |a I:(DE-He78)HD01-20160331 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|