TY  - JOUR
AU  - Seidl, Maximilian
AU  - Haghighi, Elham Bavafaye
AU  - Lösslein, Anne Kathrin
AU  - Hufnagel, Markus
AU  - Lohrmann, Florens
AU  - Schneider, Christian
AU  - Kohlfürst, Daniela S
AU  - Zenz, Werner
AU  - Gorkiewicz, Gregor
AU  - Feiterna-Sperling, Cornelia
AU  - Krüger, Renate
AU  - Bronsert, Peter
AU  - Neppl, Christina
AU  - Sommer, Kim Zoe
AU  - Stehl, Verena
AU  - Börries, Melanie
AU  - Kuntz, Martin
AU  - Henneke, Philipp
TI  - Immunopathogenic and clinical implications of advanced tissue analysis in non-tuberculous mycobacterial infections in children.
JO  - Frontiers in immunology
VL  - 16
SN  - 1664-3224
CY  - Lausanne
PB  - Frontiers Media
M1  - DKFZ-2025-01383
SP  - 1597074
PY  - 2025
AB  - Infections with non-tuberculous mycobacteria (NTM) in children usually affect the lymph nodes and surrounding tissue. Although the infection is typically self-limiting, it carries a substantial risk of complications due to persistent inflammation and invasive therapeutic interventions. Yet, the immunopathogenesis of the disease is obscure, as are biomarkers guiding treatment decisions.In this observational study, we analyzed histological samples collected in the NTMkids study to identify parameters associated with impaired wound healing and complicated disease progression. Samples from 33 patients (median age at first presentation 33 months) were investigated, with two consecutive biopsies in 9 patients.Germinal centers, a scattered distribution of granuloma associated CD4+ T-cells, higher CD8+ T-cell density inside the necrosis and foamy epitheloid cells were associated with a favorable outcome. Tissue damage presenting clinically as liquefaction was associated with an adverse outcome.The identified tissue reaction patterns in NTM infections provide insights into the biology of NTM lymphadenitis in children and may aid in more precise treatment decisions.
KW  - Humans
KW  - Mycobacterium Infections, Nontuberculous: immunology
KW  - Mycobacterium Infections, Nontuberculous: pathology
KW  - Mycobacterium Infections, Nontuberculous: microbiology
KW  - Male
KW  - Female
KW  - Child, Preschool
KW  - Infant
KW  - Child
KW  - Nontuberculous Mycobacteria: immunology
KW  - CD8-Positive T-Lymphocytes: immunology
KW  - Lymphadenitis: immunology
KW  - Lymphadenitis: pathology
KW  - Lymphadenitis: microbiology
KW  - CD4-Positive T-Lymphocytes: immunology
KW  - Lymph Nodes: pathology
KW  - Lymph Nodes: immunology
KW  - Biopsy
KW  - Germinal Center: immunology
KW  - Germinal Center: pathology
KW  - children (Other)
KW  - complications (Other)
KW  - giant cells (Other)
KW  - granuloma (Other)
KW  - histology (Other)
KW  - lymphadenitis (Other)
KW  - non-tuberculous mycobacteria (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40661952
C2  - pmc:PMC12257029
DO  - DOI:10.3389/fimmu.2025.1597074
UR  - https://inrepo02.dkfz.de/record/302843
ER  -