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@ARTICLE{Gasparin:302846,
      author       = {F. Gasparin and M. R. Tietje and E. Katab and A. Nurdinova
                      and T. Yuan and A. Chmyrov and N. Uluç and D. Jüstel and
                      F. Bassermann$^*$ and V. Ntziachristos and M. A. Pleitez},
      title        = {{L}abel-free protein-structure-sensitive live-cell
                      microscopy for patient-specific assessment of myeloma
                      therapy.},
      journal      = {Nature biomedical engineering},
      volume       = {nn},
      issn         = {2157-846X},
      address      = {Tokyo},
      publisher    = {Nature Research},
      reportid     = {DKFZ-2025-01386},
      pages        = {nn},
      year         = {2025},
      note         = {epub},
      abstract     = {The efficacy of drug therapy in multiple myeloma is
                      conventionally assessed by whole-cell-population methods,
                      serum analysis of light chains and monoclonal antibodies,
                      immunofixation electrophoresis, or by flow cytometry of bone
                      marrow aspirates and biopsies. These methods provide
                      relevant information on the presence of specific
                      immunoglobulins at high sensitivity and specificity but
                      require a large number of cells, involve long and laborious
                      sample preparation steps, and provide only tumour bulk
                      information. Here we develop a single-cell imaging technique
                      requiring a reduced number of primary cells for longitudinal
                      evaluation of patient-specific treatment and assessment of
                      treatment heterogeneity. By exploiting the mechanistic
                      action of proteasome inhibition and in synergy with the
                      label-free protein-structure specificity of mid-infrared
                      optoacoustic microscopy, we present a technology that
                      facilitates longitudinal evaluation of myeloma treatment and
                      a patient's heterogeneous response. Detecting
                      optical-generated ultrasound waves that intensify with
                      optical absorption, this technology allows observation of
                      proteins in living cells with high sensitivity.
                      Specifically, we use intermolecular β-sheet formation as a
                      biomarker for cell viability during therapy and apply it to
                      assess drug-treatment performance in multiple myeloma
                      patients.},
      cin          = {MU01},
      ddc          = {610},
      cid          = {I:(DE-He78)MU01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40659832},
      doi          = {10.1038/s41551-025-01443-3},
      url          = {https://inrepo02.dkfz.de/record/302846},
}