TY  - JOUR
AU  - Soliman, Nardine
AU  - Nedelko, Tatiana
AU  - Mandracci, Giada
AU  - Enssle, Stefan
AU  - Grass, Vincent
AU  - Fischer, Julius C
AU  - Bassermann, Florian
AU  - Poeck, Hendrik
AU  - Kobold, Sebastian
AU  - El Khawanky, Nadia
AU  - Heidegger, Simon
TI  - Targeting Intracellular Innate RNA-Sensing Systems Overcomes Resistance to CAR T-cell Therapy in Solid Tumors.
JO  - Cancer research
VL  - 85
IS  - 14
SN  - 0099-7013
CY  - Philadelphia, Pa.
PB  - AACR
M1  - DKFZ-2025-01392
SP  - 2679 - 2693
PY  - 2025
AB  - Despite the remarkable success of chimeric antigen receptor (CAR) T cells in certain hematologic malignancies, only modest responses have been achieved in solid tumors. Defective cell death pathways have recently been suggested as a tumor-intrinsic form of resistance to CAR T-cell treatment. In this study, we showed that insufficient activity of the innate RNA-sensing receptor system retinoic acid-inducible gene I (RIG-I)/mitochondrial antiviral signaling protein (MAVS) leads to tumor cell-inherent resistance to CAR T-cell attack. Active RIG-I/MAVS signaling in tumor cells primed intrinsic mitochondrial apoptosis pathways and expression of cell death receptors, which funneled into CAR T-cell-triggered cell death. CAR T-cell reliance on tumor-intrinsic RIG-I signaling was observed in various murine and human cancer types, independent of the CAR construct used, and the dependence was most pronounced under conditions with low target antigen expression or low effector/target ratios. RIG-I-induced proapoptotic priming of CAR T-cell susceptibility involved auto-/paracrine type-I IFN signaling loops and could spread to bystander tumor cells. Strong tumor-intrinsic RIG-I/MAVS signaling imprinted an activated cytolytic phenotype on tumor-interacting CAR T cells. Agonist-mediated targeting of the RIG-I pathway in the tumor microenvironment rendered murine melanoma susceptible to CAR T-cell therapy in vivo with enhanced infiltration of active CAR T cells. Together, these data identify insufficient RIG-I/MAVS activity and associated impaired cell death signaling in malignant cells as a resistance mechanism to CAR T cells. Targeting tumor-intrinsic RIG-I is a potential strategy to sensitize solid tumors to CAR T-cell treatment.Insufficient activity of the RIG-I/MAVS pathway is a tumor intrinsic resistance mechanism to CAR T cells, providing the rationale for targeting RIG-I to optimize CAR T efficacy in patients with solid cancers.
KW  - Animals
KW  - Humans
KW  - Mice
KW  - Immunotherapy, Adoptive: methods
KW  - Receptors, Chimeric Antigen: immunology
KW  - Receptors, Chimeric Antigen: metabolism
KW  - DEAD Box Protein 58: metabolism
KW  - DEAD Box Protein 58: immunology
KW  - Signal Transduction
KW  - Neoplasms: therapy
KW  - Neoplasms: immunology
KW  - Neoplasms: pathology
KW  - Adaptor Proteins, Signal Transducing: metabolism
KW  - Adaptor Proteins, Signal Transducing: immunology
KW  - Apoptosis
KW  - Immunity, Innate
KW  - Cell Line, Tumor
KW  - Tumor Microenvironment
KW  - Receptors, Immunologic
KW  - Receptors, Chimeric Antigen (NLM Chemicals)
KW  - DEAD Box Protein 58 (NLM Chemicals)
KW  - RIGI protein, human (NLM Chemicals)
KW  - Adaptor Proteins, Signal Transducing (NLM Chemicals)
KW  - MAVS protein, human (NLM Chemicals)
KW  - Receptors, Immunologic (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40305099
C2  - pmc:PMC12260516
DO  - DOI:10.1158/0008-5472.CAN-24-3425
UR  - https://inrepo02.dkfz.de/record/302852
ER  -