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@ARTICLE{Lalioti:302856,
      author       = {M.-E. Lalioti and M. C. Romero-Mulero and N. Karabacz and
                      J. Mess and H. Demollin and J. Rettkowski and K. Schuldes
                      and M. Mitterer and C. Wadle and K. Shoumariyeh$^*$ and M.
                      Egg and C. Alfonso-Gonzalez and K. Jäcklein and K.
                      Schönberger and N. Karantzelis and G. Reisig and P.
                      Aktories and I. M. Mayer and I. Tsoukala and A. Schäffer
                      and I. Tirado-Gonzalez and A. Dugourd and L. M. Braun and B.
                      Silva-Rego and M.-J. Jones and K. Kierdorf and J.
                      Saez-Rodriguez and K. Reising and S. G. Walter and H.
                      Medyouf and V. Hilgers and G. Ghiaur and R. Zeiser and D.
                      Karpova and S. Renders and S. Gravius and J. Buescher and N.
                      Cabezas-Wallscheid},
      title        = {{D}ifferentiation, ageing and leukaemia alter the metabolic
                      profile of human bone marrow haematopoietic stem and
                      progenitor cells.},
      journal      = {Nature cell biology},
      volume       = {27},
      number       = {8},
      issn         = {1465-7392},
      address      = {New York, NY},
      publisher    = {Nature America},
      reportid     = {DKFZ-2025-01396},
      pages        = {1367-1380},
      year         = {2025},
      note         = {2025 Aug;27(8):1367-1380},
      abstract     = {Metabolic cues are crucial for regulating haematopoietic
                      stem and progenitor cells (HSPCs). However, the metabolic
                      profile of human HSPCs remains poorly understood due to the
                      limited number of cells and the scarcity of bone marrow
                      samples. Here we present the integrated metabolome, lipidome
                      and transcriptome of human adult HSPCs (lineage-, CD34+,
                      CD38-) upon differentiation, ageing and acute myeloid
                      leukaemia. The combination of low-input targeted
                      metabolomics with our newly optimized low-input untargeted
                      lipidomics workflow allows us to detect up to 193
                      metabolites and lipids from a starting material of 3,000 and
                      5,000 HSPCs, respectively. Among other findings, we observe
                      elevated levels of the essential nutrient choline in HSPCs
                      compared with downstream progenitors, which decline upon
                      ageing and further decrease in acute myeloid leukaemia.
                      Functionally, we show that choline supplementation fuels
                      lipid production in HSPCs and enhances stemness. Overall,
                      our study provides a comprehensive resource identifying
                      metabolic changes that can be utilized to promote and
                      enhance human stem cell function.},
      cin          = {FR01},
      ddc          = {570},
      cid          = {I:(DE-He78)FR01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40664811},
      doi          = {10.1038/s41556-025-01709-7},
      url          = {https://inrepo02.dkfz.de/record/302856},
}