000302869 001__ 302869
000302869 005__ 20250718114119.0
000302869 0247_ $$2doi$$a10.7554/eLife.105311
000302869 0247_ $$2pmid$$apmid:40600802
000302869 0247_ $$2pmc$$apmc:PMC12221301
000302869 037__ $$aDKFZ-2025-01409
000302869 041__ $$aEnglish
000302869 082__ $$a600
000302869 1001_ $$0P:(DE-He78)4543601bf14234f35021d658a5228201$$aRoiuk, Mykola$$b0$$eFirst author$$udkfz
000302869 245__ $$aHuman eIF2A has a minimal role in translation initiation and in uORF-mediated translational control in HeLa cells.
000302869 260__ $$aCambridge$$beLife Sciences Publications$$c2025
000302869 3367_ $$2DRIVER$$aarticle
000302869 3367_ $$2DataCite$$aOutput Types/Journal article
000302869 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1752747737_14023
000302869 3367_ $$2BibTeX$$aARTICLE
000302869 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000302869 3367_ $$00$$2EndNote$$aJournal Article
000302869 500__ $$a#EA:B140#LA:B140#
000302869 520__ $$aTranslation initiation in eukaryotes requires a 40 S ribosome loaded with initiator tRNA which scans for an initiation codon. The initiator tRNA is usually recruited to the ribosome as part of a ternary complex composed of initiator tRNA, eIF2, and GTP. Although initiator tRNA recruitment was originally ascribed to another factor, eIF2A, it was later disproven and shown to occur via eIF2. Nonetheless, eIF2A is still considered a translation initiation factor because it binds the ribosome and shows genetic interactions with other initiation factors such as eIF4E. The exact function of eIF2A during translation initiation, however, remains unclear. Here, we use ribosome profiling and luciferase reporter assays to systematically test in HeLa cells the role of eIF2A in translation initiation, including translation of upstream ORFs. Since eIF2A is thought to take over the function of eIF2 when eIF2 is inhibited, we also test conditions where the integrated stress response is activated. In none of our assays, however, could we detect a role of eIF2A in translation initiation. It is possible that eIF2A plays a role in translation regulation in specific conditions that we have not tested here, or that it plays a role in a different aspect of RNA biology.
000302869 536__ $$0G:(DE-HGF)POF4-312$$a312 - Funktionelle und strukturelle Genomforschung (POF4-312)$$cPOF4-312$$fPOF IV$$x0
000302869 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000302869 650_7 $$2Other$$abiochemistry
000302869 650_7 $$2Other$$achemical biology
000302869 650_7 $$2Other$$adevelopmental biology
000302869 650_7 $$2Other$$aeIF2
000302869 650_7 $$2Other$$aeIF2A
000302869 650_7 $$2Other$$ahuman
000302869 650_7 $$2Other$$atranslation
000302869 650_7 $$2NLM Chemicals$$aEukaryotic Initiation Factor-2
000302869 650_2 $$2MeSH$$aHumans
000302869 650_2 $$2MeSH$$aHeLa Cells
000302869 650_2 $$2MeSH$$aEukaryotic Initiation Factor-2: metabolism
000302869 650_2 $$2MeSH$$aEukaryotic Initiation Factor-2: genetics
000302869 650_2 $$2MeSH$$aPeptide Chain Initiation, Translational
000302869 650_2 $$2MeSH$$aOpen Reading Frames
000302869 650_2 $$2MeSH$$aProtein Biosynthesis
000302869 650_2 $$2MeSH$$aRibosomes: metabolism
000302869 7001_ $$0P:(DE-He78)725adf28bb1f2600ee6fca8c48266e56$$aNeff, Marilena$$b1$$udkfz
000302869 7001_ $$0P:(DE-He78)5ebc16fd8019dbfde58e0125b001b599$$aTeleman, Aurelio$$b2$$eLast author$$udkfz
000302869 773__ $$0PERI:(DE-600)2687154-3$$a10.7554/eLife.105311$$gVol. 14, p. RP105311$$pRP105311$$teLife$$v14$$x2050-084X$$y2025
000302869 909CO $$ooai:inrepo02.dkfz.de:302869$$pVDB
000302869 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)4543601bf14234f35021d658a5228201$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000302869 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)725adf28bb1f2600ee6fca8c48266e56$$aDeutsches Krebsforschungszentrum$$b1$$kDKFZ
000302869 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)5ebc16fd8019dbfde58e0125b001b599$$aDeutsches Krebsforschungszentrum$$b2$$kDKFZ
000302869 9131_ $$0G:(DE-HGF)POF4-312$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vFunktionelle und strukturelle Genomforschung$$x0
000302869 9141_ $$y2025
000302869 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bELIFE : 2022$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-07-30T13:58:16Z
000302869 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-07-30T13:58:16Z
000302869 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review$$d2024-07-30T13:58:16Z
000302869 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bELIFE : 2022$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2024-12-28
000302869 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2024-12-28
000302869 9202_ $$0I:(DE-He78)B140-20160331$$kB140$$lB140 Signal Transduction in Cancer$$x0
000302869 9201_ $$0I:(DE-He78)B140-20160331$$kB140$$lB140 Signal Transduction in Cancer$$x0
000302869 9200_ $$0I:(DE-He78)B140-20160331$$kB140$$lB140 Signal Transduction in Cancer$$x0
000302869 980__ $$ajournal
000302869 980__ $$aVDB
000302869 980__ $$aI:(DE-He78)B140-20160331
000302869 980__ $$aUNRESTRICTED