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@ARTICLE{Roiuk:302869,
      author       = {M. Roiuk$^*$ and M. Neff$^*$ and A. Teleman$^*$},
      title        = {{H}uman e{IF}2{A} has a minimal role in translation
                      initiation and in u{ORF}-mediated translational control in
                      {H}e{L}a cells.},
      journal      = {eLife},
      volume       = {14},
      issn         = {2050-084X},
      address      = {Cambridge},
      publisher    = {eLife Sciences Publications},
      reportid     = {DKFZ-2025-01409},
      pages        = {RP105311},
      year         = {2025},
      note         = {#EA:B140#LA:B140#},
      abstract     = {Translation initiation in eukaryotes requires a 40 S
                      ribosome loaded with initiator tRNA which scans for an
                      initiation codon. The initiator tRNA is usually recruited to
                      the ribosome as part of a ternary complex composed of
                      initiator tRNA, eIF2, and GTP. Although initiator tRNA
                      recruitment was originally ascribed to another factor,
                      eIF2A, it was later disproven and shown to occur via eIF2.
                      Nonetheless, eIF2A is still considered a translation
                      initiation factor because it binds the ribosome and shows
                      genetic interactions with other initiation factors such as
                      eIF4E. The exact function of eIF2A during translation
                      initiation, however, remains unclear. Here, we use ribosome
                      profiling and luciferase reporter assays to systematically
                      test in HeLa cells the role of eIF2A in translation
                      initiation, including translation of upstream ORFs. Since
                      eIF2A is thought to take over the function of eIF2 when eIF2
                      is inhibited, we also test conditions where the integrated
                      stress response is activated. In none of our assays,
                      however, could we detect a role of eIF2A in translation
                      initiation. It is possible that eIF2A plays a role in
                      translation regulation in specific conditions that we have
                      not tested here, or that it plays a role in a different
                      aspect of RNA biology.},
      keywords     = {Humans / HeLa Cells / Eukaryotic Initiation Factor-2:
                      metabolism / Eukaryotic Initiation Factor-2: genetics /
                      Peptide Chain Initiation, Translational / Open Reading
                      Frames / Protein Biosynthesis / Ribosomes: metabolism /
                      biochemistry (Other) / chemical biology (Other) /
                      developmental biology (Other) / eIF2 (Other) / eIF2A (Other)
                      / human (Other) / translation (Other) / Eukaryotic
                      Initiation Factor-2 (NLM Chemicals)},
      cin          = {B140},
      ddc          = {600},
      cid          = {I:(DE-He78)B140-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40600802},
      pmc          = {pmc:PMC12221301},
      doi          = {10.7554/eLife.105311},
      url          = {https://inrepo02.dkfz.de/record/302869},
}