Home > Publications database > A translational protocol optimizes the isolation of plasma-derived extracellular vesicle proteomics. > print

001     302882
005     20250720021526.0
024 7 _ |a 10.1038/s41598-025-08366-8
|2 doi
024 7 _ |a pmid:40624148
|2 pmid
024 7 _ |a pmc:PMC12234866
|2 pmc
024 7 _ |a altmetric:179096243
|2 altmetric
037 _ _ |a DKFZ-2025-01422
041 _ _ |a English
082 _ _ |a 600
100 1 _ |a Rios de los Rios Resendiz, Jussara
|0 P:(DE-He78)f7694dce6295094b870780d683c93ed2
|b 0
|e First author
|u dkfz
245 _ _ |a A translational protocol optimizes the isolation of plasma-derived extracellular vesicle proteomics.
260 _ _ |a [London]
|c 2025
|b Springer Nature
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1752751169_14023
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
500 _ _ |a #EA:B470#LA:B470#
520 _ _ |a In translational research and clinical routine, liquid biopsy is a promising tool to direct individually targeted treatments. Among the components of liquid biopsy, extracellular vesicles (EVs) carry manyfold molecular cargo and are increasingly being studied for biomarker identification. In order to identify potential confounding factors and determine optimal conditions when studying blood-derived EV proteins, the impact of pre-analytical variables needs to be assessed. Here we establish an EV enrichment for proteomic analysis workflow in a real-world clinical setting in which we evaluate variables from blood collection through protein preparation and storage for mass spectrometry (MS). We assess hemolysis, particle concentration and size, protein quantity, protein markers and comprehensive proteomic analysis using mass spectrometry to assess the influence of different pre-analytical variables like blood collection tubes, transportation of blood samples and delayed processing. Under these conditions, density gradient and size exclusion chromatography using Sepharose CL-4B show good EV enrichment. For MS, lysis with increased protease inhibitors shows high protein yields while TCA protein precipitation results in high numbers of identified proteins. In summary, we develop here an optimized protocol for the analysis of plasma EV-derived proteomics, evaluating pre-analytical variables relevant for implementation in a clinical setting.
536 _ _ |a 312 - Funktionelle und strukturelle Genomforschung (POF4-312)
|0 G:(DE-HGF)POF4-312
|c POF4-312
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
650 _ 7 |a Extracellular vesicles
|2 Other
650 _ 7 |a Liquid biopsy
|2 Other
650 _ 7 |a Plasma
|2 Other
650 _ 7 |a Pre-analytics
|2 Other
650 _ 7 |a Proteomics
|2 Other
650 _ 7 |a Translation
|2 Other
650 _ 7 |a Blood Proteins
|2 NLM Chemicals
650 _ 7 |a Biomarkers
|2 NLM Chemicals
650 _ 7 |a Proteome
|2 NLM Chemicals
650 _ 2 |a Extracellular Vesicles: metabolism
|2 MeSH
650 _ 2 |a Extracellular Vesicles: chemistry
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Proteomics: methods
|2 MeSH
650 _ 2 |a Mass Spectrometry
|2 MeSH
650 _ 2 |a Blood Proteins
|2 MeSH
650 _ 2 |a Translational Research, Biomedical: methods
|2 MeSH
650 _ 2 |a Biomarkers: blood
|2 MeSH
650 _ 2 |a Proteome
|2 MeSH
650 _ 2 |a Liquid Biopsy: methods
|2 MeSH
700 1 _ |a Herrmann-Sim, Freya
|0 P:(DE-He78)f354c88d44ebf813c593e187da2564c9
|b 1
700 1 _ |a Wilkesmann, Liliana
|0 P:(DE-He78)a0146d1c0a5c614fe0bb012bbfea56b3
|b 2
|u dkfz
700 1 _ |a Helm, Dominic
|0 P:(DE-He78)daaed5a5b968028e6e95d273150d5ab1
|b 3
|u dkfz
700 1 _ |a Schneider, Martin
|0 P:(DE-He78)0d37cc734b95fed555f2244d6fee6320
|b 4
|u dkfz
700 1 _ |a Campione, Giorgia
|0 P:(DE-He78)7a6f726eed43caa75ce1995b0cb09a42
|b 5
|u dkfz
700 1 _ |a Plügge, Klara
|0 P:(DE-He78)6d6e51fcfc79d92b63429314280d419a
|b 6
|u dkfz
700 1 _ |a Greiner, Giovanni
|0 P:(DE-He78)d30c5d1ed305fb4a5e00c88e6842de84
|b 7
|u dkfz
700 1 _ |a Lazaro Garcia, Leonie
|0 P:(DE-He78)7aad127f8049237b8b21d1f020e476b0
|b 8
|u dkfz
700 1 _ |a Berker, Julia
|0 P:(DE-He78)bdf729ea0d3954f1b296b38db4289789
|b 9
|u dkfz
700 1 _ |a Richter, Karsten
|0 P:(DE-He78)027fe772631b4a2d7a45c439cdd75ff2
|b 10
|u dkfz
700 1 _ |a Zielske, Lin
|0 P:(DE-He78)6e1ca2b645d93cb55f1d07262b80b0f0
|b 11
700 1 _ |a Hofmann, Wolf-Karsten
|b 12
700 1 _ |a Clemm von Hohenberg, Katharina
|0 P:(DE-He78)97823de5d9dccd50446fc2389ce3ad76
|b 13
|e Last author
|u dkfz
773 _ _ |a 10.1038/s41598-025-08366-8
|g Vol. 15, no. 1, p. 24292
|0 PERI:(DE-600)2615211-3
|n 1
|p 24292
|t Scientific reports
|v 15
|y 2025
|x 2045-2322
909 C O |o oai:inrepo02.dkfz.de:302882
|p VDB
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 0
|6 P:(DE-He78)f7694dce6295094b870780d683c93ed2
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 1
|6 P:(DE-He78)f354c88d44ebf813c593e187da2564c9
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 2
|6 P:(DE-He78)a0146d1c0a5c614fe0bb012bbfea56b3
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 3
|6 P:(DE-He78)daaed5a5b968028e6e95d273150d5ab1
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 4
|6 P:(DE-He78)0d37cc734b95fed555f2244d6fee6320
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 5
|6 P:(DE-He78)7a6f726eed43caa75ce1995b0cb09a42
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 6
|6 P:(DE-He78)6d6e51fcfc79d92b63429314280d419a
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 7
|6 P:(DE-He78)d30c5d1ed305fb4a5e00c88e6842de84
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 8
|6 P:(DE-He78)7aad127f8049237b8b21d1f020e476b0
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 9
|6 P:(DE-He78)bdf729ea0d3954f1b296b38db4289789
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 10
|6 P:(DE-He78)027fe772631b4a2d7a45c439cdd75ff2
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 11
|6 P:(DE-He78)6e1ca2b645d93cb55f1d07262b80b0f0
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 13
|6 P:(DE-He78)97823de5d9dccd50446fc2389ce3ad76
913 1 _ |a DE-HGF
|b Gesundheit
|l Krebsforschung
|1 G:(DE-HGF)POF4-310
|0 G:(DE-HGF)POF4-312
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Funktionelle und strukturelle Genomforschung
|x 0
914 1 _ |y 2025
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b SCI REP-UK : 2022
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0501
|2 StatID
|b DOAJ Seal
|d 2024-07-29T15:28:26Z
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0500
|2 StatID
|b DOAJ
|d 2024-07-29T15:28:26Z
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b DOAJ : Anonymous peer review
|d 2024-07-29T15:28:26Z
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2024-12-18
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1040
|2 StatID
|b Zoological Record
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1150
|2 StatID
|b Current Contents - Physical, Chemical and Earth Sciences
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2024-12-18
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2024-12-18
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2024-12-18
915 _ _ |a IF < 5
|0 StatID:(DE-HGF)9900
|2 StatID
|d 2024-12-18
915 _ _ |a Article Processing Charges
|0 StatID:(DE-HGF)0561
|2 StatID
|d 2024-12-18
915 _ _ |a Fees
|0 StatID:(DE-HGF)0700
|2 StatID
|d 2024-12-18
920 2 _ |0 I:(DE-He78)B470-20160331
|k B470
|l Translationale Lymphomforschung
|x 0
920 1 _ |0 I:(DE-He78)B470-20160331
|k B470
|l Translationale Lymphomforschung
|x 0
920 1 _ |0 I:(DE-He78)W120-20160331
|k W120
|l Proteomics
|x 1
920 1 _ |0 I:(DE-He78)W230-20160331
|k W230
|l Elektronenmikroskopie
|x 2
920 0 _ |0 I:(DE-He78)B470-20160331
|k B470
|l Translationale Lymphomforschung
|x 0
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a I:(DE-He78)B470-20160331
980 _ _ |a I:(DE-He78)W120-20160331
980 _ _ |a I:(DE-He78)W230-20160331
980 _ _ |a UNRESTRICTED

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21