%0 Journal Article
%A Beumer, Niklas
%A Imbusch, Charles D
%A Kaufmann, Tamara
%A Schmidleithner, Lisa
%A Gütter, Kathrin
%A Stüve, Philipp
%A Marchel, Harriet
%A Weichenhan, Dieter
%A Bähr, Marion
%A Ruhland, Brigitte
%A Marini, Federico
%A Sanderink, Lieke
%A Ritter, Uwe
%A Simon, Malte
%A Braband, Kathrin Luise
%A Voss, Morten Michael
%A Helbich, Sara Salome
%A Mihoc, Delia Mihaela
%A Hotz-Wagenblatt, Agnes
%A Nassabi, Hadrian
%A Eigenberger, Andreas
%A Prantl, Lukas
%A Gebhard, Claudia
%A Rehli, Michael
%A Strieder, Nicholas
%A Singh, Kartikeya
%A Schmidl, Christian
%A Plass, Christoph
%A Huehn, Jochen
%A Hehlgans, Thomas
%A Polansky, Julia K
%A Brors, Benedikt
%A Delacher, Michael
%A Feuerer, Markus
%T DNA hypomethylation traits define human regulatory T cells in cutaneous tissue and identify their blood recirculating counterparts.
%J Nature immunology
%V 26
%N 8
%@ 1529-2908
%C London
%I Springer Nature Limited
%M DKFZ-2025-01438
%P 1315-1328
%D 2025
%Z #EA:B330#LA:B330# / 2025 Aug;26(8):1315-1328
%X CD4+ regulatory T (Treg) cells in tissues play crucial immunoregulatory and regenerative roles. Despite their importance, the epigenetics and differentiation of human tissue Treg cells are incompletely understood. Here, we performed genome-wide DNA methylation analysis of human Treg cells from skin and blood and integrated these data into a multiomic framework, including chromatin accessibility and gene expression. This analysis identified programs that governed the tissue adaptation of skin Treg cells. We found that subfamilies of transposable elements represented a major constituent of the hypomethylated landscape in tissue Treg cells. Based on T cell antigen receptor sequence and DNA hypomethylation homologies, our data indicate that blood CCR8+ Treg cells contain recirculating human skin Treg cells. Conversely, differences in chromatin accessibility and gene expression suggest a certain reversal of the tissue adaptation program during recirculation. Our findings provide insights into the biology of human tissue Treg cells, which may help harness these cells for therapeutic purposes.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40670618
%R 10.1038/s41590-025-02210-x
%U https://inrepo02.dkfz.de/record/302991