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000303003 1001_ $$aDulovic, Alex$$b0
000303003 245__ $$aLongitudinal analysis of humoral and cellular immunity in SARS-CoV-2 exposed families.
000303003 260__ $$a[London]$$bSpringer Nature$$c2025
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000303003 520__ $$aIdentification of previous SARS-CoV-2 infection typically relies on serology, yet T-cells play a key role in the adaptive immune response against SARS-CoV-2. Here, we investigated in parallel the SARS-CoV-2-specific as well as endemic human coronavirus-specific humoral and cross-reactive cellular responses in children and adults. We analyzed clinical data and blood samples from a family cohort of 96 children and 144 adults at 3-4 and 11-12 months after their first contact with SARS-CoV-2. Humoral response was assessed by a multiplex immunoassay with high sensitivity and specificity (MULTICOV-AB). Cellular responses were analyzed by IFN-γ ELISPOT using four different established epitope compositions (ECs) to discriminate between SARS-CoV-2 specific and HCoV cross-reactive T-cell responses. While the majority of adults had a combined serological and T-cell response, relatively more children had a T-cell response alone rather than a combined response. The magnitude of the T-cell response correlated with symptoms and the humoral response. In addition, SARS-CoV-2 infection significantly boosted the endemic coronavirus-specific cellular response. Overall, our data suggest discordant humoral and cellular responses, reflecting either abortive infection, cellular sensitization with rapid viral clearance or rapid antibody waning or a combination of these phenomena. Restricting epidemiologic analysis to SARS-CoV-2 serological data may underestimate rates of infection with or at least exposure to SARS-CoV-2 in children.
000303003 536__ $$0G:(DE-HGF)POF4-314$$a314 - Immunologie und Krebs (POF4-314)$$cPOF4-314$$fPOF IV$$x0
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000303003 650_7 $$2NLM Chemicals$$aAntibodies, Viral
000303003 650_2 $$2MeSH$$aHumans
000303003 650_2 $$2MeSH$$aCOVID-19: immunology
000303003 650_2 $$2MeSH$$aImmunity, Humoral
000303003 650_2 $$2MeSH$$aSARS-CoV-2: immunology
000303003 650_2 $$2MeSH$$aAdult
000303003 650_2 $$2MeSH$$aImmunity, Cellular
000303003 650_2 $$2MeSH$$aMale
000303003 650_2 $$2MeSH$$aFemale
000303003 650_2 $$2MeSH$$aChild
000303003 650_2 $$2MeSH$$aLongitudinal Studies
000303003 650_2 $$2MeSH$$aAntibodies, Viral: blood
000303003 650_2 $$2MeSH$$aAntibodies, Viral: immunology
000303003 650_2 $$2MeSH$$aMiddle Aged
000303003 650_2 $$2MeSH$$aChild, Preschool
000303003 650_2 $$2MeSH$$aT-Lymphocytes: immunology
000303003 650_2 $$2MeSH$$aAdolescent
000303003 650_2 $$2MeSH$$aYoung Adult
000303003 650_2 $$2MeSH$$aInfant
000303003 650_2 $$2MeSH$$aCross Reactions
000303003 7001_ $$0P:(DE-He78)e946c6dc2b4cb5a11f86daef065b425f$$aRabsteyn, Armin$$b1
000303003 7001_ $$aRemppis, Jonathan$$b2
000303003 7001_ $$aGentzcke, Irene K E$$b3
000303003 7001_ $$aMueller, Julia$$b4
000303003 7001_ $$aTuecks, Nadja$$b5
000303003 7001_ $$aBecker, Matthias$$b6
000303003 7001_ $$aJunker, Daniel$$b7
000303003 7001_ $$aKaiser, Philipp D$$b8
000303003 7001_ $$aTraenkle, Bjoern$$b9
000303003 7001_ $$aRothbauer, Ulrich$$b10
000303003 7001_ $$0P:(DE-He78)aa8b428f7b1df6694113a5a7d3c4832a$$aWalz, Juliane S$$b11$$udkfz
000303003 7001_ $$aPeter, Andreas$$b12
000303003 7001_ $$aHörber, Sebastian$$b13
000303003 7001_ $$aGanzenmueller, Tina$$b14
000303003 7001_ $$aIftner, Thomas$$b15
000303003 7001_ $$0P:(DE-He78)ec362aaba198f7b2bc8ccd436181f827$$aStich, Maximilian$$b16$$udkfz
000303003 7001_ $$aTönshoff, Burkhard$$b17
000303003 7001_ $$aHenneke, Philipp$$b18
000303003 7001_ $$aElling, Roland$$b19
000303003 7001_ $$aDebatin, Klaus-Michael$$b20
000303003 7001_ $$aJanda, Ales$$b21
000303003 7001_ $$aSchneiderhan-Marra, Nicole$$b22
000303003 7001_ $$aFranz, Axel R$$b23
000303003 7001_ $$0P:(DE-HGF)0$$aLang, Peter$$b24
000303003 7001_ $$aRenk, Hanna$$b25
000303003 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-025-07739-3$$gVol. 15, no. 1, p. 26041$$n1$$p26041$$tScientific reports$$v15$$x2045-2322$$y2025
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