%0 Journal Article
%A Morales, Ivonne
%A Bueggeln, Josh
%A Denzler, Anna
%A Sonntag-Buck, Vera
%A Börner, Kathleen
%A Chlanda, Petr
%A Koeppel, Lisa
%A Deckert, Andreas
%A Bärnighausen, Till
%A Knop, Michael
%A Denkinger, Claudia M
%T Performance and feasibility of self-microsampling of capillary blood and saliva for serological testing of SARS-CoV-2.
%J PLOS ONE
%V 20
%N 7
%@ 1932-6203
%C San Francisco, California, US
%I PLOS
%M DKFZ-2025-01458
%P e0327821
%D 2025
%X Serology is a valuable tool to estimate infections, case-fatality rates, and immunity. However, venipuncture and clinical infrastructure hinder scalability. This study evaluated the performance, feasibility and user experience of using a microsampling device for self-collected capillary blood and saliva to determine total SARS-CoV-2 S RBD antibodies.It included 149 participants with (n = 48) or without (n = 101) a known history of SARS-CoV-2 infection and confirmed antibodies. Venous and capillary blood were self- or professionally collected from all, and saliva was self-collected by 46/48 participants with antibodies. The detection of SARS-CoV-2 S RBD antibodies in all sample types was tested using a high-throughput platform and agreement was calculated. Intra- and inter-rater agreement between serum and capillary blood in participants with an unknown antibody status was also assessed. Participants evaluated the device's user-friendliness through questionnaires.Among the 48 participants with known past infection and antibodies, agreement was 100
%K Humans
%K Saliva: virology
%K COVID-19: diagnosis
%K COVID-19: blood
%K COVID-19: virology
%K Female
%K SARS-CoV-2: immunology
%K SARS-CoV-2: isolation & purification
%K Male
%K Middle Aged
%K Antibodies, Viral: blood
%K Antibodies, Viral: analysis
%K Adult
%K Feasibility Studies
%K COVID-19 Serological Testing: methods
%K Aged
%K Specimen Handling: methods
%K Blood Specimen Collection: methods
%K Capillaries
%K Antibodies, Viral (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40644438
%2 pmc:PMC12250565
%R 10.1371/journal.pone.0327821
%U https://inrepo02.dkfz.de/record/303011