TY  - JOUR
AU  - Morales, Ivonne
AU  - Bueggeln, Josh
AU  - Denzler, Anna
AU  - Sonntag-Buck, Vera
AU  - Börner, Kathleen
AU  - Chlanda, Petr
AU  - Koeppel, Lisa
AU  - Deckert, Andreas
AU  - Bärnighausen, Till
AU  - Knop, Michael
AU  - Denkinger, Claudia M
TI  - Performance and feasibility of self-microsampling of capillary blood and saliva for serological testing of SARS-CoV-2.
JO  - PLOS ONE
VL  - 20
IS  - 7
SN  - 1932-6203
CY  - San Francisco, California, US
PB  - PLOS
M1  - DKFZ-2025-01458
SP  - e0327821
PY  - 2025
AB  - Serology is a valuable tool to estimate infections, case-fatality rates, and immunity. However, venipuncture and clinical infrastructure hinder scalability. This study evaluated the performance, feasibility and user experience of using a microsampling device for self-collected capillary blood and saliva to determine total SARS-CoV-2 S RBD antibodies.It included 149 participants with (n = 48) or without (n = 101) a known history of SARS-CoV-2 infection and confirmed antibodies. Venous and capillary blood were self- or professionally collected from all, and saliva was self-collected by 46/48 participants with antibodies. The detection of SARS-CoV-2 S RBD antibodies in all sample types was tested using a high-throughput platform and agreement was calculated. Intra- and inter-rater agreement between serum and capillary blood in participants with an unknown antibody status was also assessed. Participants evaluated the device's user-friendliness through questionnaires.Among the 48 participants with known past infection and antibodies, agreement was 100
KW  - Humans
KW  - Saliva: virology
KW  - COVID-19: diagnosis
KW  - COVID-19: blood
KW  - COVID-19: virology
KW  - Female
KW  - SARS-CoV-2: immunology
KW  - SARS-CoV-2: isolation & purification
KW  - Male
KW  - Middle Aged
KW  - Antibodies, Viral: blood
KW  - Antibodies, Viral: analysis
KW  - Adult
KW  - Feasibility Studies
KW  - COVID-19 Serological Testing: methods
KW  - Aged
KW  - Specimen Handling: methods
KW  - Blood Specimen Collection: methods
KW  - Capillaries
KW  - Antibodies, Viral (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40644438
C2  - pmc:PMC12250565
DO  - DOI:10.1371/journal.pone.0327821
UR  - https://inrepo02.dkfz.de/record/303011
ER  -