% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @ARTICLE{Logsdon:303081, author = {G. A. Logsdon and P. Ebert and P. A. Audano and M. Loftus and D. Porubsky and J. Ebler and F. Yilmaz and P. Hallast and T. Prodanov and D. Yoo and C. A. Paisie and W. T. Harvey and X. Zhao and G. V. Martino and M. Henglin and K. M. Munson and K. Rabbani and C.-S. Chin and B. Gu and H. Ashraf and S. Scholz and O. Austine-Orimoloye and P. Balachandran and M. J. Bonder$^*$ and H. Cheng and Z. Chong and J. Crabtree and M. Gerstein and L. A. Guethlein and P. Hasenfeld and G. Hickey and K. Hoekzema and S. E. Hunt and M. Jensen and Y. Jiang and S. Koren and Y. Kwon and C. Li and H. Li and J. Li and P. J. Norman and K. K. Oshima and B. Paten and A. M. Phillippy and N. R. Pollock and T. Rausch and M. Rautiainen and Y. Song and A. Söylev and A. Sulovari and L. Surapaneni and V. Tsapalou and W. Zhou and Y. Zhou and Q. Zhu and M. C. Zody and R. E. Mills and S. E. Devine and X. Shi and M. E. Talkowski and M. J. P. Chaisson and A. T. Dilthey and M. K. Konkel and J. O. Korbel and C. Lee and C. R. Beck and E. E. Eichler and T. Marschall}, title = {{C}omplex genetic variation in nearly complete human genomes.}, journal = {Nature}, volume = {644}, number = {8076}, issn = {0028-0836}, address = {London [u.a.]}, publisher = {Nature Publ. Group}, reportid = {DKFZ-2025-01506}, pages = {430-441}, year = {2025}, note = {Division of Computational Genomics and Systems Genetics, German Cancer Research Center, Heidelberg, Germany. / 2025 Aug;644(8076):430-441}, abstract = {Diverse sets of complete human genomes are required to construct a pangenome reference and to understand the extent of complex structural variation. Here we sequence 65 diverse human genomes and build 130 haplotype-resolved assemblies (median continuity of 130 Mb), closing $92\%$ of all previous assembly gaps1,2 and reaching telomere-to-telomere status for $39\%$ of the chromosomes. We highlight complete sequence continuity of complex loci, including the major histocompatibility complex (MHC), SMN1/SMN2, NBPF8 and AMY1/AMY2, and fully resolve 1,852 complex structural variants. In addition, we completely assemble and validate 1,246 human centromeres. We find up to 30-fold variation in α-satellite higher-order repeat array length and characterize the pattern of mobile element insertions into α-satellite higher-order repeat arrays. Although most centromeres predict a single site of kinetochore attachment, epigenetic analysis suggests the presence of two hypomethylated regions for $7\%$ of centromeres. Combining our data with the draft pangenome reference1 significantly enhances genotyping accuracy from short-read data, enabling whole-genome inference3 to a median quality value of 45. Using this approach, 26,115 structural variants per individual are detected, substantially increasing the number of structural variants now amenable to downstream disease association studies.}, cin = {B260}, ddc = {500}, cid = {I:(DE-He78)B260-20160331}, pnm = {312 - Funktionelle und strukturelle Genomforschung (POF4-312)}, pid = {G:(DE-HGF)POF4-312}, typ = {PUB:(DE-HGF)16}, pubmed = {pmid:40702183}, doi = {10.1038/s41586-025-09140-6}, url = {https://inrepo02.dkfz.de/record/303081}, }