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@ARTICLE{AbuAhmad:303114,
author = {M. Abu Ahmad and O. Radinsky and B. Kaufman and K. Waidha
and E. Gharra and S. Dim and D. B. Manikandan and N. Ofir
and D. Jäger$^*$ and M. Meyer$^*$ and M. Elkabets and K. S.
Campbell and M. Zektser and R. Gazit and O. Rouvio and F.
Momburg$^*$ and A. Porgador},
title = {{HLA}-{E}[p{HLA}-{G}] {C}omplex {S}pecific {M}onoclonal
{A}ntibody {E}nhancing {NK} {A}ctivity in {M}ultiple
{M}yeloma.},
journal = {Blood advances},
volume = {nn},
issn = {2473-9529},
address = {Washington, DC},
publisher = {American Society of Hematology},
reportid = {DKFZ-2025-01533},
pages = {nn},
year = {2025},
note = {epub},
abstract = {HLA-E presenting the HLA-G leader peptide VMAPRTLFL
(HLA-E[pHLA-G]) on tumor cells plays a crucial role in
suppressing natural killer(NK) and cytotoxic CD8+ T cells
through NKG2A interaction. While blocking HLA-E:NKG2A is a
promising immune checkpoint(IC) approach in cancer therapy,
toxicity remains a major clinical concern. We developed a
novel immune checkpoint inhibitor(ICI) that selectively
prevents HLA-E:NKG2A interaction, a monoclonal antibody(mAb)
that selectively targets the HLA-E[pHLA-G] complex,
distinguishing cancerous from non-cancerous cells. In
clinical bone marrow samples from multiple myeloma(MM)
patients, 4D7 specifically recognized tumor-associated
HLA-E-peptide complexes. Using NK cells from healthy donors,
4D7 effectively blocked the HLA-E:NKG2A interaction and
enhanced NKG2A-positive NK cell activity in autologous MM
cell co-cultures. Importantly, 4D7 did not inhibit
NKG2C-positive NK cells, preserving their activity. Even
though NKG2C also interacts with HLA-E. In MM-bearing mice
treated with human NK cells, 4D7 significantly reduced tumor
growth. This targeted approach activates NK cells only
against tumor cells presenting HLA-E-peptide complexes,
potentially minimizing toxicity compared to current NKG2A
inhibitors. The development of 4D7 highlights a promising
advancement in immunotherapy for hematological malignancies,
offering improved outcomes for MM patients and a foundation
for broader application across cancer types.},
cin = {D120},
ddc = {610},
cid = {I:(DE-He78)D120-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40706037},
doi = {10.1182/bloodadvances.2025016276},
url = {https://inrepo02.dkfz.de/record/303114},
}