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@ARTICLE{Guo:303249,
      author       = {W. Guo and I. Kaczmarczyk and K. Kopietz and F. Flegler and
                      S. Russo and E. Cigirgan and A. Chramiec-Głąbik and Ł.
                      Koziej and C. Cirzi$^*$ and J. Peschek and K. Reuter and M.
                      Helm and S. Glatt and F. Tuorto},
      title        = {{Q}ueuosine is incorporated into precursor t{RNA} before
                      splicing.},
      journal      = {Nature Communications},
      volume       = {16},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2025-01594},
      pages        = {7044},
      year         = {2025},
      note         = {DKFZ-ZMBH Alliance},
      abstract     = {Each newly transcribed tRNA molecule must undergo
                      processing and receive modifications to become functional.
                      Queuosine (Q) is a tRNA modification present at position 34
                      of four tRNAs with 'GUN' anticodons. Among these, the
                      precursor of tRNATyr carries an intronic sequence within the
                      anticodon loop that is removed by an essential non-canonical
                      splicing event. The functional and temporal coupling between
                      tRNA-splicing and Q-incorporation remains elusive. Here, we
                      demonstrate in vitro and in vivo that intron-containing
                      precursors of tRNATyr are modified with Q or with the
                      Q-derivative galactosyl-queuosine (galQ) before being
                      spliced. We show that this order of events is conserved in
                      mouse, human, flies and worms. Using single particle
                      cryo-EM, we confirm that pre-tRNATyr is a bona fide
                      substrate of the QTRT1/2 complex, which catalyzes the
                      incorporation of Q into the tRNA. Our results elucidate the
                      hierarchical interplay that coordinates Q-incorporation and
                      splicing in eukaryotic tRNAs, providing a relevant but
                      unappreciated aspect of the cellular tRNA maturation
                      process.},
      keywords     = {Animals / RNA Splicing / Nucleoside Q: metabolism / Humans
                      / Mice / RNA Precursors: metabolism / RNA Precursors:
                      genetics / RNA, Transfer: metabolism / RNA, Transfer:
                      genetics / Cryoelectron Microscopy / Introns: genetics /
                      Anticodon: genetics / Caenorhabditis elegans: genetics /
                      Pentosyltransferases: metabolism / Nucleoside Q (NLM
                      Chemicals) / RNA Precursors (NLM Chemicals) / RNA, Transfer
                      (NLM Chemicals) / Anticodon (NLM Chemicals) /
                      Pentosyltransferases (NLM Chemicals)},
      cin          = {A130},
      ddc          = {500},
      cid          = {I:(DE-He78)A130-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40745156},
      doi          = {10.1038/s41467-025-62220-z},
      url          = {https://inrepo02.dkfz.de/record/303249},
}