Home > Publications database > Queuosine is incorporated into precursor tRNA before splicing. > print

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024 7 _ |a 10.1038/s41467-025-62220-z
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100 1 _ |a Guo, Wei
|0 0009-0009-1463-3099
|b 0
245 _ _ |a Queuosine is incorporated into precursor tRNA before splicing.
260 _ _ |a [London]
|c 2025
|b Springer Nature
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520 _ _ |a Each newly transcribed tRNA molecule must undergo processing and receive modifications to become functional. Queuosine (Q) is a tRNA modification present at position 34 of four tRNAs with 'GUN' anticodons. Among these, the precursor of tRNATyr carries an intronic sequence within the anticodon loop that is removed by an essential non-canonical splicing event. The functional and temporal coupling between tRNA-splicing and Q-incorporation remains elusive. Here, we demonstrate in vitro and in vivo that intron-containing precursors of tRNATyr are modified with Q or with the Q-derivative galactosyl-queuosine (galQ) before being spliced. We show that this order of events is conserved in mouse, human, flies and worms. Using single particle cryo-EM, we confirm that pre-tRNATyr is a bona fide substrate of the QTRT1/2 complex, which catalyzes the incorporation of Q into the tRNA. Our results elucidate the hierarchical interplay that coordinates Q-incorporation and splicing in eukaryotic tRNAs, providing a relevant but unappreciated aspect of the cellular tRNA maturation process.
536 _ _ |a 311 - Zellbiologie und Tumorbiologie (POF4-311)
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650 _ 7 |a Nucleoside Q
|0 57072-36-3
|2 NLM Chemicals
650 _ 7 |a RNA Precursors
|2 NLM Chemicals
650 _ 7 |a RNA, Transfer
|0 9014-25-9
|2 NLM Chemicals
650 _ 7 |a Anticodon
|2 NLM Chemicals
650 _ 7 |a Pentosyltransferases
|0 EC 2.4.2.-
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a RNA Splicing
|2 MeSH
650 _ 2 |a Nucleoside Q: metabolism
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a RNA Precursors: metabolism
|2 MeSH
650 _ 2 |a RNA Precursors: genetics
|2 MeSH
650 _ 2 |a RNA, Transfer: metabolism
|2 MeSH
650 _ 2 |a RNA, Transfer: genetics
|2 MeSH
650 _ 2 |a Cryoelectron Microscopy
|2 MeSH
650 _ 2 |a Introns: genetics
|2 MeSH
650 _ 2 |a Anticodon: genetics
|2 MeSH
650 _ 2 |a Caenorhabditis elegans: genetics
|2 MeSH
650 _ 2 |a Pentosyltransferases: metabolism
|2 MeSH
700 1 _ |a Kaczmarczyk, Igor
|0 0000-0002-5607-7416
|b 1
700 1 _ |a Kopietz, Kevin
|0 0009-0007-0423-4049
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700 1 _ |a Flegler, Florian
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700 1 _ |a Russo, Stefano
|0 0000-0001-7539-0427
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700 1 _ |a Cigirgan, Ege
|b 5
700 1 _ |a Chramiec-Głąbik, Andrzej
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700 1 _ |a Koziej, Łukasz
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700 1 _ |a Cirzi, Cansu
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700 1 _ |a Peschek, Jirka
|0 0000-0001-8158-9301
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700 1 _ |a Reuter, Klaus
|b 10
700 1 _ |a Helm, Mark
|0 0000-0002-0154-0928
|b 11
700 1 _ |a Glatt, Sebastian
|0 0000-0003-2815-7133
|b 12
700 1 _ |a Tuorto, Francesca
|b 13
773 _ _ |a 10.1038/s41467-025-62220-z
|g Vol. 16, no. 1, p. 7044
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