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@ARTICLE{Chen:303258,
author = {W. W. Chen and T. A. Rodrigues and D. Wendscheck and A. G.
Pedrosa and C. Yang and T. Francisco and T.
Möcklinghoff$^*$ and A. Zografakis and B. Nunes-Silva and
R. E. Avraham and A. R. Silva and M. J. Ferreira and H. Das
and J. Koster and S. Neuwirth and J. Bender and S.
Oeljeklaus and V. Sondhi and C. Gatsogiannis and M.
Schuldiner and E. Zalckvar and K. Hofmann and H. R. Waterham
and R. J. DeBerardinis and J. E. Azevedo and B. Warscheid},
title = {{PEX}39 facilitates the peroxisomal import of
{PTS}2-containing proteins.},
journal = {Nature cell biology},
volume = {27},
number = {8},
issn = {1465-7392},
address = {New York, NY},
publisher = {Nature America},
reportid = {DKFZ-2025-01603},
pages = {1256-1271},
year = {2025},
note = {2025 Aug;27(8):1256-1271},
abstract = {Peroxisomes are metabolic organelles essential for human
health. Defects in peroxisomal biogenesis proteins (also
known as peroxins (PEXs)) cause devastating disease. PEX7
binds proteins containing a type 2 peroxisomal targeting
signal (PTS2) to enable their import from the cytosol into
peroxisomes, although many aspects of this process remain
enigmatic. Utilizing in vitro assays, yeast and human cells,
we show that PEX39, a previously uncharacterized protein, is
a cytosolic peroxin that facilitates the import of
PTS2-containing proteins by binding PEX7 and stabilizing its
interaction with cargo proteins containing a PTS2. PEX39 and
PEX13, a peroxisomal membrane translocon protein, both
possess an (R/K)PWE motif necessary for PEX7 binding.
Handover of PEX7 from PEX39 to PEX13 via these motifs
provides a new paradigm for peroxisomal protein import and
biogenesis. Collectively, this work reveals how PEX39 and
(R/K)PWE motifs facilitate the import of PTS2-containing
proteins and advances our understanding of peroxisomal
disease.},
cin = {B200},
ddc = {570},
cid = {I:(DE-He78)B200-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40739340},
doi = {10.1038/s41556-025-01711-z},
url = {https://inrepo02.dkfz.de/record/303258},
}