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@ARTICLE{Ebert:303362,
      author       = {B. C. Ebert and I. C. MacArthur and H. C. Ketchum and M.
                      Musheev and C. Niehrs$^*$ and M. Suzuki and M. M. Dawlaty},
      title        = {{E}stablishment of neuronal and glial competence of neural
                      stem cells requires distinct enzymatic activities of {TET}
                      enzymes.},
      journal      = {Stem cell reports},
      volume       = {nn},
      issn         = {2213-6711},
      address      = {Maryland Heights, MO},
      publisher    = {Cell Press},
      reportid     = {DKFZ-2025-01612},
      pages        = {nn},
      year         = {2025},
      note         = {DKFZ-ZMBH Alliance / epub},
      abstract     = {Ten-eleven translocation (TET1/2/3) enzymes are expressed
                      in neural stem cells (NSCs). They iteratively oxidize
                      5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC),
                      5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). The
                      significance of hydroxymethylation (i.e., 5hmC) versus
                      formylation and carboxylation (i.e., active demethylation)
                      is undefined. We generated NSCs lacking only TET formylation
                      and carboxylation activities (Tet-TFoCa) and compared them
                      to NSCs lacking all three TET activities (Tet-TMut).
                      Tet-TFoCa NSCs could differentiate into neurons but not into
                      glial cells, while Tet-TMut NSCs could not form either cell
                      type. Mechanistically, neuronal genes retained 5hmC at their
                      enhancers in Tet-TFoCa NSCs and were expressed normally,
                      consistent with the ability of these cells to form neurons.
                      In contrast, enhancers of glial genes were hypermethylated
                      in both Tet-TFoCa and Tet-TMut NSCs underpinning
                      downregulation of these genes and the glial block in these
                      cells. Our findings implicate TET-driven hydroxymethylation
                      in establishing NSC neuronal competence and formylation and
                      carboxylation in defining NSC glial competence.},
      keywords     = {5caC (Other) / 5fC (Other) / 5hmC (Other) / DNA
                      demethylation (Other) / Neural stem cells (Other) / TET
                      enzymes (Other) / glial (Other) / neurons (Other)},
      cin          = {A050},
      ddc          = {610},
      cid          = {I:(DE-He78)A050-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40749667},
      doi          = {10.1016/j.stemcr.2025.102595},
      url          = {https://inrepo02.dkfz.de/record/303362},
}