TY  - JOUR
AU  - Vega-Rubin-de-Celis, Silvia
AU  - Kristani, Amanda
AU  - Kudla, Matthias
AU  - Mergener, Svenja
AU  - Corrochano-Ruiz, Andrés
AU  - Larafa, Safa
AU  - Montero-Vergara, Jetsy
AU  - Ahle, Laura-Marie
AU  - Will, Rainer
AU  - Lever, Mael
AU  - Grünwald, Viktor
AU  - Hadaschik, Boris
AU  - Jendrossek, Verena
AU  - Bechrakis, Nikolaos E
AU  - Pena-Llopis, Samuel
TI  - Autophagy suppression via SRC induction represents a therapeutic vulnerability for BAP1-mutant cancers.
JO  - Autophagy
VL  - 21
IS  - 12
SN  - 1554-8627
CY  - Abingdon, Oxon
PB  - Taylor & Francis
M1  - DKFZ-2025-01614
SP  - 3324-3343
PY  - 2025
N1  - #LA:B330# / 2025 Dec;21(12):3324-3343
AB  - BAP1 is a tumor suppressor and epigenetic modifier that is frequently mutated in cancer, leading to increased aggressiveness and metastasis, as well as poor patient survival. Unfortunately, there are currently no specific therapies for metastatic tumors harboring BAP1 mutations. In this study, we have identified a new targetable BAP1-associated autophagic vulnerability. We demonstrate that BAP1 transcriptionally regulates the proto-oncogene SRC, a non-receptor tyrosine kinase. SRC then binds to, phosphorylates, and inactivates BECN1 (Beclin 1), an essential autophagy protein. This inhibits autophagy in cells derived from various cancer types with BAP1 mutations. Treatment of these cells with SRC inhibitors (such as dasatinib, bosutinib and saracatinib) and autophagy-inducing drugs (such as Tat-BECN1, SW076956 and SW063058) demonstrated a synergistic interaction between these compounds both in vitro and in ovo using a chick Chorioallantoic Membrane (CAM) assay. Furthermore, ex vivo studies employing patient-derived tumor organoids (PDTOs) of uveal melanoma (UM) and clear-cell renal cell carcinoma (ccRCC) as preclinical models have substantiated the synergism of these drugs, preferentially in the context of BAP1 loss. Our findings elucidate a novel BAP1-SRC-BECN1-autophagy regulatory axis that can be exploited therapeutically in precision oncology through the combination of SRC inhibitors and autophagy inducers, contingent upon patient stratification for BAP1 loss.Significance: Deadly cancers with BAP1 mutations suppress autophagy by phosphorylating the autophagy regulator BECN1 via the proto-oncogene SRC. Treatment with SRC inhibitors and autophagy inducers exhibited synergism in vitro, in ovo and in patient-derived tumor organoids with BAP1 loss, paving the way for treating BAP1-deficient cancers with autophagy inducers and kinase inhibitors.
KW  - BRCA1-associated protein 1 (Other)
KW  - PDOs (Other)
KW  - cholangiocarcinoma (Other)
KW  - patient-derived organoids (Other)
KW  - personalized medicine (Other)
KW  - tumoroids (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40754831
DO  - DOI:10.1080/15548627.2025.2535265
UR  - https://inrepo02.dkfz.de/record/303364
ER  -