%0 Journal Article
%A Schmitz, Daniel
%A Prax, Stefan
%A Kliment, Martin
%A Gocke, Felix
%A Kazdal, Daniel
%A Allgäuer, Michael
%A Penzel, Roland
%A Kirchner, Martina
%A Neumann, Olaf
%A Sültmann, Holger
%A Budczies, Jan
%A Schirmacher, Peter
%A Bergmann, Frank
%A Ritz, Jörg-Peter
%A Hinze, Raoul
%A Grassmann, Felix
%A Rudi, Jochen
%A Stenzinger, Albrecht
%A Volckmar, Anna-Lena
%T Differentiating Main-Duct IPMN from Chronic Pancreatitis Using Next-Generation Sequencing of Main Pancreatic Duct Fluid: A Pilot Study.
%J Diagnostics
%V 15
%N 15
%@ 2075-4418
%C Basel
%I MDPI
%M DKFZ-2025-01686
%P 1964
%D 2025
%X Background: A dilated main pancreatic duct (MPD) ≥ 5 mm can be observed in main-duct IPMNs (MD-IPMN) and chronic pancreatitis (CP); however, distinguishing between the two differently treated diseases can be difficult. Cell-free (cf) DNA in MPD fluid obtained by EUS-guided FNA might help to distinguish MD-IPMN from CP. Methods: All patients with a dilated MPD ≥ 5 mm on EUS during the period of 1 June 2017 to 30 April 2024 were prospectively analysed in this single-centre study, with EUS-guided MPD fluid aspiration performed for suspected MD-IPMN or CP in patients who were suitable for surgery. Twenty-two known gastrointestinal cancer genes, including GNAS and KRAS, were analysed by deep targeted (dt) NGS. The results were correlated with resected tissue, biopsy, and long-term follow-up. Results: A total of 164 patients with a dilated MPD were identified, of which 30 (18.3
%K chronic (Other)
%K endoscopic ultrasound-guided fine needle aspiration (Other)
%K high-throughput nucleotide sequencing (Other)
%K intraductal papillary mucinous neoplasm (Other)
%K pancreatitis (Other)
%K prospective study (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:40804928
%R 10.3390/diagnostics15151964
%U https://inrepo02.dkfz.de/record/303495