TY  - JOUR
AU  - Schmitz, Daniel
AU  - Prax, Stefan
AU  - Kliment, Martin
AU  - Gocke, Felix
AU  - Kazdal, Daniel
AU  - Allgäuer, Michael
AU  - Penzel, Roland
AU  - Kirchner, Martina
AU  - Neumann, Olaf
AU  - Sültmann, Holger
AU  - Budczies, Jan
AU  - Schirmacher, Peter
AU  - Bergmann, Frank
AU  - Ritz, Jörg-Peter
AU  - Hinze, Raoul
AU  - Grassmann, Felix
AU  - Rudi, Jochen
AU  - Stenzinger, Albrecht
AU  - Volckmar, Anna-Lena
TI  - Differentiating Main-Duct IPMN from Chronic Pancreatitis Using Next-Generation Sequencing of Main Pancreatic Duct Fluid: A Pilot Study.
JO  - Diagnostics
VL  - 15
IS  - 15
SN  - 2075-4418
CY  - Basel
PB  - MDPI
M1  - DKFZ-2025-01686
SP  - 1964
PY  - 2025
AB  - Background: A dilated main pancreatic duct (MPD) ≥ 5 mm can be observed in main-duct IPMNs (MD-IPMN) and chronic pancreatitis (CP); however, distinguishing between the two differently treated diseases can be difficult. Cell-free (cf) DNA in MPD fluid obtained by EUS-guided FNA might help to distinguish MD-IPMN from CP. Methods: All patients with a dilated MPD ≥ 5 mm on EUS during the period of 1 June 2017 to 30 April 2024 were prospectively analysed in this single-centre study, with EUS-guided MPD fluid aspiration performed for suspected MD-IPMN or CP in patients who were suitable for surgery. Twenty-two known gastrointestinal cancer genes, including GNAS and KRAS, were analysed by deep targeted (dt) NGS. The results were correlated with resected tissue, biopsy, and long-term follow-up. Results: A total of 164 patients with a dilated MPD were identified, of which 30 (18.3
KW  - chronic (Other)
KW  - endoscopic ultrasound-guided fine needle aspiration (Other)
KW  - high-throughput nucleotide sequencing (Other)
KW  - intraductal papillary mucinous neoplasm (Other)
KW  - pancreatitis (Other)
KW  - prospective study (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40804928
DO  - DOI:10.3390/diagnostics15151964
UR  - https://inrepo02.dkfz.de/record/303495
ER  -