000303649 001__ 303649
000303649 005__ 20250824022536.0
000303649 0247_ $$2doi$$a10.1038/s41467-025-61500-y
000303649 0247_ $$2pmid$$apmid:40817089
000303649 0247_ $$2pmc$$apmc:PMC12356879
000303649 0247_ $$2altmetric$$aaltmetric:180346002
000303649 037__ $$aDKFZ-2025-01713
000303649 041__ $$aEnglish
000303649 082__ $$a500
000303649 1001_ $$aWruck, Florian$$b0
000303649 245__ $$aCo-translational ribosome pairing enables native assembly of misfolding-prone subunits.
000303649 260__ $$a[London]$$bSpringer Nature$$c2025
000303649 3367_ $$2DRIVER$$aarticle
000303649 3367_ $$2DataCite$$aOutput Types/Journal article
000303649 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1755759558_3179
000303649 3367_ $$2BibTeX$$aARTICLE
000303649 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000303649 3367_ $$00$$2EndNote$$aJournal Article
000303649 500__ $$aA250 30.09.2023 geschlossen
000303649 520__ $$aProtein complexes are pivotal to most cellular processes. Emerging evidence indicating dimer assembly by pairs of ribosomes suggests yet unknown folding mechanisms involving two nascent chains. Here, we show that co-translational ribosome pairing allows their nascent chains to 'chaperone each other', thus enabling the formation of coiled-coil homodimers from subunits that misfold individually. We developed an integrated single-molecule fluorescence and force spectroscopy approach to probe the folding and assembly of two nascent chains extending from nearby ribosomes, using the intermediate filament lamin as a model system. Ribosome proximity during early translation stages is found to be critical: when interactions between nascent chains are inhibited or delayed, they become trapped in stable misfolded states that are no longer assembly-competent. Conversely, early interactions allow the two nascent chains to nucleate native-like quaternary structures that grow in size and stability as translation advances. We conjecture that protein folding mechanisms enabled by ribosome cooperation are more broadly relevant to intermediate filaments and other protein classes.
000303649 536__ $$0G:(DE-HGF)POF4-319H$$a319H - Addenda (POF4-319H)$$cPOF4-319H$$fPOF IV$$x0
000303649 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000303649 7001_ $$00000-0001-6701-7112$$aSchmitt, Jaro$$b1
000303649 7001_ $$aTill, Katharina$$b2
000303649 7001_ $$aFenzl, Kai$$b3
000303649 7001_ $$aBertolini, Matilde$$b4
000303649 7001_ $$aTippmann, Frank$$b5
000303649 7001_ $$00000-0002-1785-1659$$aKatranidis, Alexandros$$b6
000303649 7001_ $$0P:(DE-He78)9d539bc25fa8f4ff093b6f6e10d39476$$aBukau, Bernd$$b7$$udkfz
000303649 7001_ $$aKramer, Günter$$b8
000303649 7001_ $$aTans, Sander J$$b9
000303649 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-025-61500-y$$gVol. 16, no. 1, p. 7626$$n1$$p7626$$tNature Communications$$v16$$x2041-1723$$y2025
000303649 909CO $$ooai:inrepo02.dkfz.de:303649$$pVDB
000303649 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)9d539bc25fa8f4ff093b6f6e10d39476$$aDeutsches Krebsforschungszentrum$$b7$$kDKFZ
000303649 9131_ $$0G:(DE-HGF)POF4-319H$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vAddenda$$x0
000303649 9141_ $$y2025
000303649 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNAT COMMUN : 2022$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-01-30T07:48:07Z
000303649 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-01-30T07:48:07Z
000303649 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Peer review$$d2024-01-30T07:48:07Z
000303649 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ$$d2024-01-30T07:48:07Z
000303649 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)9915$$2StatID$$aIF >= 15$$bNAT COMMUN : 2022$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2025-01-02
000303649 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2025-01-02
000303649 9201_ $$0I:(DE-He78)Z999-20160331$$kZ999$$lErimitus im DKFZ$$x0
000303649 980__ $$ajournal
000303649 980__ $$aVDB
000303649 980__ $$aI:(DE-He78)Z999-20160331
000303649 980__ $$aUNRESTRICTED