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@ARTICLE{Quiroz:303955,
      author       = {J. N. Quiroz and M. Sielaff and D. Kondrateva and F.
                      Boukhallouk and G. J. Godoy and C. R. Molina and B. Moonen
                      and C. C. Motran and J. Bogie and H. D. Luján and S.
                      Tenzer$^*$ and T. Sparwasser and L. Berod},
      title        = {{TLR}9-{D}riven {S}-{P}almitoylation in {D}endritic {C}ells
                      {R}eveals {I}mmune and {M}etabolic {P}rotein {T}argets.},
      journal      = {European journal of immunology},
      volume       = {55},
      number       = {8},
      issn         = {0014-2980},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {DKFZ-2025-01730},
      pages        = {e70039},
      year         = {2025},
      abstract     = {Dendritic cells (DCs) rely on Toll-like receptor 9 (TLR9)
                      to detect unmethylated CpG motifs in microbial DNA,
                      triggering essential immune responses. While the downstream
                      signaling pathways of TLR9 activation are well
                      characterized, their impact on S-palmitoylation is unknown.
                      S-palmitoylation, involving the reversible attachment of
                      palmitic acid to cysteine residues, plays a crucial role in
                      regulating protein function and is catalyzed by the ZDHHC
                      family of palmitoyl-acyltransferases (PATs). In this study,
                      we investigated the S-palmitoylated proteome of bone
                      marrow-derived GM-CSF DCs (GM-DCs) at resting and following
                      TLR9 activation with CpGB. Using the
                      click-chemistry-compatible analog 17-octadecynoic acid
                      (17-ODYA) and mass spectrometry (MS)-based proteomics, we
                      characterized dynamic remodeling of S-palmitoylation in
                      response to TLR9 activation. This included enrichment of
                      targets involved in immune and metabolic pathways.
                      Transcriptomic analysis of mice and human DCs revealed
                      TLR9-driven modulation of PAT-encoding genes. Subsequently,
                      we explored the contribution of Zdhhc9 expression to the
                      regulation of S-palmitoylation in DCs. Using gene knockout
                      approaches, we identified candidate protein targets
                      potentially linked to ZDHHC9 activity. Interestingly,
                      modulation of Zdhhc9 expression alone did not influence DC
                      maturation, suggesting that other PATs might compensate for
                      its activity. Together, our findings reveal a novel layer of
                      regulation in TLR9 signaling mediated by S-palmitoylation.},
      keywords     = {S‐palmitoylation (Other) / TLR9 signaling (Other) /
                      dendritic cells (Other) / innate immunity (Other)},
      cin          = {D191},
      ddc          = {610},
      cid          = {I:(DE-He78)D191-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40828036},
      pmc          = {pmc:PMC12363430},
      doi          = {10.1002/eji.70039},
      url          = {https://inrepo02.dkfz.de/record/303955},
}