000303972 001__ 303972
000303972 005__ 20250824022552.0
000303972 0247_ $$2doi$$a10.1039/D5LC00287G
000303972 0247_ $$2pmid$$apmid:40833685
000303972 0247_ $$2pmc$$apmc:PMC12366776
000303972 0247_ $$2ISSN$$a1473-0197
000303972 0247_ $$2ISSN$$a1473-0189
000303972 0247_ $$2altmetric$$aaltmetric:180335965
000303972 037__ $$aDKFZ-2025-01741
000303972 041__ $$aEnglish
000303972 082__ $$a540
000303972 1001_ $$00009-0006-6418-7582$$aRuider, Iris$$b0
000303972 245__ $$aAccelerated maturation of branched organoids confined in collagen droplets.
000303972 260__ $$aCambridge$$bRSC$$c2025
000303972 3367_ $$2DRIVER$$aarticle
000303972 3367_ $$2DataCite$$aOutput Types/Journal article
000303972 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1755862842_12287
000303972 3367_ $$2BibTeX$$aARTICLE
000303972 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000303972 3367_ $$00$$2EndNote$$aJournal Article
000303972 500__ $$aepub
000303972 520__ $$aDroplet-based organoid culture offers several advantages over conventional bulk organoid culture, such as improved yield, reproducibility, and throughput. However, organoids grown in droplets typically display only a spherical geometry and lack the intricate structural complexity found in native tissue. By incorporating singularised pancreatic ductal adenocarcinoma cells into collagen droplets, we achieve the growth of branched structures, indicating a more complex interaction with the surrounding hydrogel. A comparison of organoid growth in droplets of different diameters showed that while geometrical confinement improves organoid homogeneity, it also impairs the formation of more complex organoid morphologies. Thus, only in 750 μm diameter collagen droplets did we achieve the consistent growth of highly branched structures with a morphology closely resembling the structural complexity achieved in traditional bulk organoid culture. Moreover, our analysis of organoid morphology and transcriptomic data suggests an accelerated maturation of organoids cultured in collagen droplets, highlighting a shift in developmental timing compared to traditional systems.
000303972 536__ $$0G:(DE-HGF)POF4-899$$a899 - ohne Topic (POF4-899)$$cPOF4-899$$fPOF IV$$x0
000303972 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000303972 7001_ $$aPastucha, Anna$$b1
000303972 7001_ $$00009-0008-2500-4928$$aRaich, Marion K$$b2
000303972 7001_ $$aXu, Wentao$$b3
000303972 7001_ $$aLiu, Yan$$b4
000303972 7001_ $$0P:(DE-HGF)0$$aReichert, Maximilian$$b5
000303972 7001_ $$00000-0001-6678-5208$$aWeitz, David$$b6
000303972 7001_ $$00000-0002-4608-9544$$aBausch, Andreas R$$b7
000303972 773__ $$0PERI:(DE-600)2056646-3$$a10.1039/D5LC00287G$$gp. 10.1039.D5LC00287G$$pnn$$tLab on a chip$$vnn$$x1473-0197$$y2025
000303972 909CO $$ooai:inrepo02.dkfz.de:303972$$pVDB
000303972 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-HGF)0$$aDeutsches Krebsforschungszentrum$$b5$$kDKFZ
000303972 9131_ $$0G:(DE-HGF)POF4-899$$1G:(DE-HGF)POF4-890$$2G:(DE-HGF)POF4-800$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000303972 9141_ $$y2025
000303972 915__ $$0StatID:(DE-HGF)0430$$2StatID$$aNational-Konsortium$$d2024-12-05$$wger
000303972 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bLAB CHIP : 2022$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-05
000303972 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bLAB CHIP : 2022$$d2024-12-05
000303972 9201_ $$0I:(DE-He78)MU01-20160331$$kMU01$$lDKTK Koordinierungsstelle München$$x0
000303972 980__ $$ajournal
000303972 980__ $$aVDB
000303972 980__ $$aI:(DE-He78)MU01-20160331
000303972 980__ $$aUNRESTRICTED