TY  - JOUR
AU  - Deshpande, Anushka
AU  - Weirauch, Leo
AU  - Baral, Tapan Kumar
AU  - Steier, Marco
AU  - Borlepawar, Ankush
AU  - Kumari, Manju
AU  - Kilian, Lucia S
AU  - Richter, Karsten
AU  - Hammer, Elke
AU  - Frank, Derk
AU  - Schmidt, Constanze
AU  - Frey, Norbert
AU  - Rangrez, Ashraf Y
TI  - Elevated levels of Letm1 drives mitochondrial dysfunction and cardiomyocyte stress-mediated apoptosis in cultured cardiomyocytes.
JO  - Cell communication and signaling
VL  - 23
IS  - 1
SN  - 1478-811X
CY  - London
PB  - Biomed Central
M1  - DKFZ-2025-01759
SP  - 378
PY  - 2025
AB  - Cardiac ischemia, a predominant cause of heart failure, is marked by profound mitochondrial dysfunction, dysregulated ion homeostasis, and maladaptive cellular remodeling, all of which compromise cardiac performance. The mitochondrial inner membrane protein Leucine zipper-EF-hand containing Transmembrane Protein 1 (Letm1), implicated in Wolf-Hirschhorn Syndrome, is essential for mitochondrial function. Although genetic alterations in Letm1 are linked to cardiomyopathies, its specific contributions to cardiac pathophysiology, particularly in the context of ischemic heart disease, remain poorly defined. This study aims to elucidate the role of Letm1 in ischemic cardiac pathology and its mechanistic impact on cardiomyocyte function.Letm1 expression was assessed in human and murine models of heart failure due to ischemic cardiomyopathy (ICM) and cardiac hypertrophy. Letm1 was overexpressed in neonatal rat ventricular cardiomyocytes, adult mouse cardiomyocytes, and human induced pluripotent stem cell (iPSC)-derived cardiomyocytes to study mitochondrial function (Seahorse assays), structural and molecular remodeling (fluorescence microscopy, transmission electron microscopy (TEM), qPCR, immunoblotting), transcriptomic/proteomic profiles, calcium handling and electrophysiology (patch-clamp), autophagic flux (Bafilomycin A1, LC3-RFP-GFP), and cell survival.Letm1 was markedly upregulated in ICM in both human and murine hearts, but unchanged in hypertrophic heart failure. Overexpression of Letm1 in cardiomyocytes resulted in profound mitochondrial dysfunction, including downregulation of oxidative phosphorylation (OXPHOS) genes, impaired membrane potential, reduced ATP output, increased proton leak, and elevated ROS levels. A metabolic shift toward glycolysis was observed, accompanied by reduced fatty acid oxidation. Electron microscopy revealed mitochondrial fragmentation, mitophagic vesicles, and sarcomeric disarray. Transcriptomic and proteomic analyses highlighted dysregulation of genes linked to mitochondrial organization, ion transport, and autophagy. Electrophysiologically, Letm1 reduced L-type Ca2+ current density and significantly shortened action potential duration, leading to impaired contractility. Letm1 overexpression activated upstream autophagy regulators (AMPK, ULK1) and enhanced LC3-II and p62 accumulation, but autophagic flux was impaired, as confirmed by LC3-RFP-GFP reporter and exacerbated by Bafilomycin A1 treatment. This dysregulated autophagy was coupled with mitochondrial stress, increased apoptosis (cleaved caspases), and reduced cardiomyocyte viability.This study indicates that Letm1 upregulation drives mitochondrial dysfunction, electrophysiology alterations, and activation of autophagy and apoptosis, culminating in cardiomyocyte injury in ischemic cardiomyopathy. By disrupting OXPHOS, calcium handling, and cell survival pathways, Letm1 contributes to ischemic remodeling and cardiac dysfunction. Targeting Letm1 presents a promising therapeutic strategy to alleviate ischemic damage and preserve cardiac function.
KW  - Myocytes, Cardiac: metabolism
KW  - Myocytes, Cardiac: pathology
KW  - Animals
KW  - Humans
KW  - Apoptosis
KW  - Mice
KW  - Rats
KW  - Mitochondria: metabolism
KW  - Mitochondria: pathology
KW  - Cells, Cultured
KW  - Membrane Proteins: metabolism
KW  - Membrane Proteins: genetics
KW  - Autophagy
KW  - Calcium-Binding Proteins: metabolism
KW  - Calcium-Binding Proteins: genetics
KW  - Induced Pluripotent Stem Cells: metabolism
KW  - Male
KW  - Arrhythmias (Other)
KW  - Cardiomyocytes (Other)
KW  - Hypertrophy (Other)
KW  - Letm1 (Other)
KW  - Mitochondrial metabolism (Other)
KW  - LETM1 protein, human (NLM Chemicals)
KW  - Membrane Proteins (NLM Chemicals)
KW  - Calcium-Binding Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40849623
DO  - DOI:10.1186/s12964-025-02378-7
UR  - https://inrepo02.dkfz.de/record/304091
ER  -