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@ARTICLE{Heuser:304269,
      author       = {E. Heuser and A. Ebinger and S. Holtfreter and S. A. Wolf
                      and A. E. Zautner and R. Ryll and S. Drewes and B. Matzkeit
                      and B. Hoffmann and D. Höper and M. Keller and A. Groseth
                      and G. Wilharm and D. M. Mrochen and A. Obiegala and F. Doss
                      and C. Mehl and T. Eisenberg and S. Niendorf and S.
                      Böttcher and A. Karger and C. Schröder and E. Ehrke-Schulz
                      and K. Schmidt$^*$ and M. Beer and M. H. Groschup and T.
                      Semmler and G. Heckel and M. Pfeffer and C. Wylezich and R.
                      G. Ulrich},
      title        = {{A}pplication of a comprehensive approach to pathogen
                      screening in a stowaway rat on an airplane.},
      journal      = {Scientific reports},
      volume       = {15},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2025-01810},
      pages        = {31963},
      year         = {2025},
      abstract     = {In April 2017, a rat was observed on an airplane during a
                      flight from Miami (USA) to Berlin (Germany). After landing
                      in Berlin, significant efforts were made to trap the rat and
                      disinfect the airplane. As rats are known reservoir hosts
                      for a variety of zoonotic pathogens, this event necessitated
                      the establishment of a standard workflow for the detection
                      of rodent-borne pathogens. Tissue and blood samples were
                      collected to screen for zoonotic pathogens and other known
                      and novel infectious agents using an array of open-view
                      methods (cultivation and characterization of bacteria,
                      high-throughput sequencing) and pathogen-specific methods
                      (e.g. PCR, RT-PCR and multiplex serology). The black rat
                      (Rattus rattus), as confirmed by mtDNA sequences, carried
                      several infectious agents. Cultivation experiments revealed
                      the presence of seven bacterial and two fungal genera. In
                      addition, a methicillin-susceptible Staphylococcus aureus
                      strain of MLST-CC45 was detected by culture-based
                      approaches, and its full genome was sequenced.
                      High-throughput sequencing identified novel picobirnaviruses
                      and various bacterial genera, the majority of which
                      represent commensals rather than pathogens. Despite the
                      diversity of bacterial, viral, and fungal species that can
                      be expected in wild rats, only a few zoonotic and
                      non-zoonotic pathogens were detected in the stowaway rat.
                      Nonetheless, this incident highlights the potential of
                      international (and cross-continental) dissemination of
                      pathogens and the need for a standardized workflow to
                      provide comprehensive coverage of the diversity of
                      microorganisms in such animals.},
      keywords     = {Animals / Rats: microbiology / Aircraft / Bacteria:
                      genetics / Bacteria: isolation $\&$ purification / Bacteria:
                      classification / High-Throughput Nucleotide Sequencing /
                      Zoonoses: microbiology / Staphylococcus aureus (Other) /
                      Commensals (Other) / High-throughput sequencing (Other) /
                      Multiplex serological assay (Other) / Pathogen importation
                      (Other) / Picobirnavirus (Other)},
      cin          = {W440},
      ddc          = {600},
      cid          = {I:(DE-He78)W440-20160331},
      pnm          = {319H - Addenda (POF4-319H)},
      pid          = {G:(DE-HGF)POF4-319H},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40885807},
      doi          = {10.1038/s41598-025-13199-6},
      url          = {https://inrepo02.dkfz.de/record/304269},
}