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@ARTICLE{Yuan:304295,
      author       = {C. Yuan$^*$ and A. Neuner and J. Streubel$^*$ and A.
                      Bhanushali and M. Simons and S. P. Acebrón and G.
                      Pereira$^*$},
      title        = {{T}he interplay between {W}nt and m{TOR} signaling
                      modulates ciliogenesis in human retinal epithelial cells.},
      journal      = {PLoS biology},
      volume       = {23},
      number       = {9},
      issn         = {1544-9173},
      address      = {Lawrence, KS},
      publisher    = {PLoS},
      reportid     = {DKFZ-2025-01831},
      pages        = {e3003369 -},
      year         = {2025},
      note         = {DKFZ-ZMBH Alliance / #EA:A180#LA:A180#},
      abstract     = {The primary cilium is a microtubule-based organelle
                      essential for various cellular functions, particularly
                      signal transduction. While the role of cilia in regulating
                      signaling pathways has been extensively studied, the impact
                      of signaling pathways on cilia formation remains less well
                      understood. Wnt signals are critical modulators of cell
                      fate. In this study, we investigate how modulating Wnt
                      signaling affects cilia formation in human retinal pigment
                      epithelial (hTERT-RPE1) cells. Our findings show that
                      enhancement of Wnt/LRP6 signaling before serum starvation
                      delays ciliogenesis. Cells with high baseline Wnt activity
                      exhibited distal appendage dysregulation, failure to remove
                      CP110/CEP97 from mother centrioles, and reduced Rab8-vesicle
                      docking, which are critical events for cilia membrane
                      establishment and axoneme extension. Additionally, these
                      cells displayed reduced autophagic flux, increased mTOR
                      kinase activity, and elevated OFD1 levels at centriolar
                      satellites. Importantly, mTOR inhibition rescued
                      ciliogenesis in cells with elevated Wnt activity,
                      underscoring the interplay between these signaling pathways.
                      Our data also indicate that insufficient Wnt signaling
                      activation disrupts ciliogenesis, emphasizing the need for
                      precisely regulated Wnt levels.},
      cin          = {A180},
      ddc          = {610},
      cid          = {I:(DE-He78)A180-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40892854},
      doi          = {10.1371/journal.pbio.3003369},
      url          = {https://inrepo02.dkfz.de/record/304295},
}