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@ARTICLE{Kaur:304477,
author = {S. Kaur and B. Wenzel and R. Oehme and C. Wiesner and K.
Kopka$^*$ and R.-P. Moldovan},
title = {{T}he hydrogenation side-reaction in copper-mediated
radiofluorination.},
journal = {EJNMMI radiopharmacy and chemistry},
volume = {10},
number = {1},
issn = {2365-421X},
address = {[Cham, Switzerland]},
publisher = {Springer International Publishing},
reportid = {DKFZ-2025-01869},
pages = {60},
year = {2025},
abstract = {Copper-mediated radiofluorination (CMRF) is a breakthrough
in 18F-radiochemistry, enabling 18F incorporation into
molecules even at electron-rich aromatic positions. In
recent years, several improved protocols have been reported
to advance the application of CMRF. These advancements
primarily focus on improving radiochemical conversion,
expanding substrate scope, and enabling scalability for
remote-controlled radiotracer production. Despite these
improvements, one major challenge remains: the
protodemetallation. Protodemetallation is a common side
reaction in transition metal-mediated cross-couplings that
takes place by a mechanism that is not yet fully elucidated.
In 18F-chemistry, the formation of the hydrogenated side
product (HSP) can interfere with the chromatographic
purification of the desired radiotracer, resulting in
complex radiotracer production.The present work investigates
the factors influencing the rate of the hydrogenation
reaction as well as the source of hydrogen in the CMRF by
use of model precursors bearing -B(OH)2, -Bpin, -BEpin and
-SnBu3 as leaving groups. While the CMRF reactions are
usually carried out under anhydrous conditions, the
formation rate of the HSP was evaluated by controlling the
chemical constituents (type and molarity of reagents) as
well as the physical parameters (time and temperature).
Moreover, experiments with deuterated reagents complemented
by high-resolution mass spectrometry (HRMS) analysis were
carried out to identify the source of hydrogen for the
reductive elimination step.This study identifies reaction
parameters that influence hydrogenation side reactions in
CMRF, enabling high RCC with minimal HSP formation. The
optimal reaction conditions include low temperature, short
reaction time, and minimal amount of precursor, copper, and
ideally no base and alcohols as solvents. Among the
evaluated precursors, -BEpin afforded the lowest HSP
formation, while -B(OH)2 afforded the highest. Overall, this
study showed that the selection of proper reaction reagents
and the fine-tuning of reaction parameters can substantially
reduce the HSP formation while maintaining optimal
radiochemical conversion.},
keywords = {BEpin (Other) / CMRF (Other) / Copper-mediated
radiofluorination (Other) / Fluorine-18 (Other) /
Hydrogenated side product (Other) / Protodeboronation
(Other)},
cin = {DD01},
ddc = {610},
cid = {I:(DE-He78)DD01-20160331},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40921858},
pmc = {pmc:PMC12417351},
doi = {10.1186/s41181-025-00384-1},
url = {https://inrepo02.dkfz.de/record/304477},
}