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000304483 1001_ $$aMansouri, Siavash$$b0
000304483 245__ $$aLiving human lung slices for ex vivo modelling of lung cancer.
000304483 260__ $$aAnn Arbor, Michigan$$bJCI Insight$$c2025
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000304483 520__ $$aThe tumor microenvironment (TME) markedly affects cancer progression, yet traditional animal models do not fully recapitulate the situation in humans. To address this, we developed tumor-derived precision lung slices (TD-PCLS), an ex vivo platform for studying the lung TME and evaluating therapies. TD-PCLS, viable for 8-10 days, preserve the heterogeneity and metabolic activity of primary tumors, as confirmed by seahorse analysis. Using multispectral FACS and phenocycler multiplex imaging, we spatially profiled TME components and cancer cell functionality. Additionally, TD-PCLS revealed patient-specific responses to chemo- and immunotherapies. To complement TD-PCLS, we established tumor-cell-seeded PCLS (TCS-PCLS) by introducing tumor and immune cells into healthy lung slices. This model highlighted macrophage-tumor interactions as critical for tumor cell proliferation, migration, and immune modulation. Together, these platforms provide a robust tool for lung cancer research, enabling precision medicine and advancing therapeutic discovery.
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000304483 650_7 $$2Other$$aImmunology
000304483 650_7 $$2Other$$aImmunotherapy
000304483 650_7 $$2Other$$aLung cancer
000304483 650_7 $$2Other$$aOncology
000304483 650_2 $$2MeSH$$aHumans
000304483 650_2 $$2MeSH$$aLung Neoplasms: pathology
000304483 650_2 $$2MeSH$$aLung Neoplasms: immunology
000304483 650_2 $$2MeSH$$aLung Neoplasms: therapy
000304483 650_2 $$2MeSH$$aTumor Microenvironment: immunology
000304483 650_2 $$2MeSH$$aLung: pathology
000304483 650_2 $$2MeSH$$aLung: immunology
000304483 650_2 $$2MeSH$$aCell Proliferation
000304483 650_2 $$2MeSH$$aCell Line, Tumor
000304483 650_2 $$2MeSH$$aMacrophages: immunology
000304483 650_2 $$2MeSH$$aCell Movement
000304483 7001_ $$aKarger, Annika$$b1
000304483 7001_ $$aRuppert, Clemens$$b2
000304483 7001_ $$aSchneider, Marc A$$b3
000304483 7001_ $$aWeigert, Andreas$$b4
000304483 7001_ $$aNandigama, Rajender$$b5
000304483 7001_ $$aAliraj, Blerina$$b6
000304483 7001_ $$0P:(DE-He78)b0217be22a29dfdb8927f227016fd33b$$aStrotmann, Lisa$$b7$$udkfz
000304483 7001_ $$aCherian, Anoop V$$b8
000304483 7001_ $$aPruefer, Diethard$$b9
000304483 7001_ $$aDorfmuller, Peter$$b10
000304483 7001_ $$aFink, Ludger$$b11
000304483 7001_ $$aAlkoudmani, Ibrahim$$b12
000304483 7001_ $$aGattenlöhner, Stefan$$b13
000304483 7001_ $$aEul, Bastian$$b14
000304483 7001_ $$aAlthoff, Andre$$b15
000304483 7001_ $$aKleine, Peter$$b16
000304483 7001_ $$aWinter, Hauke$$b17
000304483 7001_ $$aGuenther, Andreas$$b18
000304483 7001_ $$aGhofrani, Hossein-Ardeschir$$b19
000304483 7001_ $$aPullamsetti, Soni S$$b20
000304483 7001_ $$aGrimminger, Friedrich$$b21
000304483 7001_ $$aSeeger, Werner$$b22
000304483 7001_ $$aSavai, Rajkumar$$b23
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