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@ARTICLE{Mansouri:304483,
      author       = {S. Mansouri and A. Karger and C. Ruppert and M. A.
                      Schneider and A. Weigert and R. Nandigama and B. Aliraj and
                      L. Strotmann$^*$ and A. V. Cherian and D. Pruefer and P.
                      Dorfmuller and L. Fink and I. Alkoudmani and S.
                      Gattenlöhner and B. Eul and A. Althoff and P. Kleine and H.
                      Winter and A. Guenther and H.-A. Ghofrani and S. S.
                      Pullamsetti and F. Grimminger and W. Seeger and R. Savai},
      title        = {{L}iving human lung slices for ex vivo modelling of lung
                      cancer.},
      journal      = {JCI insight},
      volume       = {10},
      number       = {17},
      issn         = {2379-3708},
      address      = {Ann Arbor, Michigan},
      publisher    = {JCI Insight},
      reportid     = {DKFZ-2025-01875},
      pages        = {e190703},
      year         = {2025},
      abstract     = {The tumor microenvironment (TME) markedly affects cancer
                      progression, yet traditional animal models do not fully
                      recapitulate the situation in humans. To address this, we
                      developed tumor-derived precision lung slices (TD-PCLS), an
                      ex vivo platform for studying the lung TME and evaluating
                      therapies. TD-PCLS, viable for 8-10 days, preserve the
                      heterogeneity and metabolic activity of primary tumors, as
                      confirmed by seahorse analysis. Using multispectral FACS and
                      phenocycler multiplex imaging, we spatially profiled TME
                      components and cancer cell functionality. Additionally,
                      TD-PCLS revealed patient-specific responses to chemo- and
                      immunotherapies. To complement TD-PCLS, we established
                      tumor-cell-seeded PCLS (TCS-PCLS) by introducing tumor and
                      immune cells into healthy lung slices. This model
                      highlighted macrophage-tumor interactions as critical for
                      tumor cell proliferation, migration, and immune modulation.
                      Together, these platforms provide a robust tool for lung
                      cancer research, enabling precision medicine and advancing
                      therapeutic discovery.},
      keywords     = {Humans / Lung Neoplasms: pathology / Lung Neoplasms:
                      immunology / Lung Neoplasms: therapy / Tumor
                      Microenvironment: immunology / Lung: pathology / Lung:
                      immunology / Cell Proliferation / Cell Line, Tumor /
                      Macrophages: immunology / Cell Movement / Immunology (Other)
                      / Immunotherapy (Other) / Lung cancer (Other) / Oncology
                      (Other)},
      cin          = {B200},
      ddc          = {610},
      cid          = {I:(DE-He78)B200-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40728884},
      doi          = {10.1172/jci.insight.190703},
      url          = {https://inrepo02.dkfz.de/record/304483},
}